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PPAR alpha抗体

KO/KD Orthogonal Overexpression
Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098
Anti-PPAR alpha antibody used in ChIP assay (ChIP assay). GTX101098
Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX101098
Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX101098
Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098
Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098
Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098

目录号 GTX101098

文献引用 24
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P, ChIP assay

种属反应

 Human, Mouse, Rat, Honeybee, Queen Bee, Plant
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P Assay dependent
ChIP assay Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

52 kDa. ( Note )

预测种属反应

Bovine, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human PPAR alpha. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2037654

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

peroxisome proliferator activated receptor alpha , NR1C1 , PPAR , PPARalpha , hPPAR

功能与背景

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098

GTX101098 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPAR alpha antibody used in ChIP assay (ChIP assay). GTX101098

GTX101098 ChIP assay Image

Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either control rabbit IgG or anti-PPAR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.
ChIP experiment and primer designs are based on J Biol Chem. 2013 Jan 18;288(3):1726-38.

Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX101098

GTX101098 IHC-P Image

PPAR alpha antibody detects PPAR alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PPAR alpha stained by PPAR alpha antibody (GTX101098) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX101098

GTX101098 IHC-P Image

PPAR alpha antibody detects PPAR alpha protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PPAR alpha stained by PPAR alpha antibody (GTX101098) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098

GTX101098 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPAR alpha antibody (GTX101098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098

GTX101098 WB Image

Orthogonal

The data was published in the 2017 in Sci Rep. PMID: 28169366

Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098

GTX101098 WB Image

The data was published in the 2022 in Commun Biol. PMID: 35552531

文献引用

Application Reference

Shou JW et al. Int J Mol Sci 2023; 24 (14) Berberine Reduces Lipid Accumulation in Obesity via Mediating Transcriptional Function of PPARδ.
Saito K et al. J Biol Chem 2023; 299 (7) : 104890 Obesity-induced metabolic imbalance allosterically modulates CtBP2 to inhibit PPAR-alpha transcriptional activity.
Egusa G et al. iScience 2023; 26 (7) : 107143 Selective activation of PPARα maintains thermogenic capacity of beige adipocytes.
Guo X et al. iScience 2023; 26 (12) : 108446 Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions.
Takuro Toda et al. Commun Biol 2022; 5 (1) : 453 Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria.
实验应用 : WB
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产品评论

PPAR alpha antibody 目录号 GTX101098

Rating
( Average 5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098
Date : Anonymous submitted on 23-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 50 μg
Blocking : Odyssey blocking buffer, 20°C, 1Hr
Primary Antibody : 1:500, 20°C, 1Hr
Figure Note : The band intensity of PPAR alpha was increased by transfection of PPAR alpha plasmid, and was decreased by transfection of siRNA for PPAR alpha.
Anti-PPAR alpha antibody used in Western Blot (WB). GTX101098
Date : Anonymous submitted on 20-May-2021
Score :
Application Tested : WB
Sample : Brown adipocytes extract
Amount used : 50 μg
Blocking : 5% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Figure Note : CL: CL316243, adrenergic-beta 3 receptor agonist
我要评论产品
Application Reference
Shou JW et al. Int J Mol Sci 2023; 24 (14) Berberine Reduces Lipid Accumulation in Obesity via Mediating Transcriptional Function of PPARδ.
Saito K et al. J Biol Chem 2023; 299 (7):104890 Obesity-induced metabolic imbalance allosterically modulates CtBP2 to inhibit PPAR-alpha transcriptional activity.
Egusa G et al. iScience 2023; 26 (7):107143 Selective activation of PPARα maintains thermogenic capacity of beige adipocytes.
Guo X et al. iScience 2023; 26 (12):108446 Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions.
Takuro Toda et al. Commun Biol 2022; 5 (1):453 Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria.
实验应用 : WB
Lin CH et al. Nutrients 2021; 13 (11) Consumption of Non-Nutritive Sweetener, Acesulfame Potassium Exacerbates Atherosclerosis through Dysregulation of Lipid Metabolism in ApoE-/- Mice.
实验应用 : WB
种属反应 : Mouse
Chen HJ et al. Evid Based Complement Alternat Med 2021; 2021:9967910 Alleviative Effect of Ruellia tuberosa L. on Insulin Resistance and Abnormal Lipid Accumulation in TNF-α-Treated FL83B Mouse Hepatocytes.
实验应用 : WB
种属反应 : Plant
Igarashi Y et al. Biomed Pharmacother 2021; 140:111714 Glavonoid-rich oil supplementation reduces stearoyl-coenzyme A desaturase 1 expression and improves systemic metabolism in diabetic, obese KK-Ay mice.
实验应用 : WB
Dai J et al. Elife 2021; 10 Curcumin promotes AApoAII amyloidosis and peroxisome proliferation in mice by activating the PPARα signaling pathway.
实验应用 : WB, IHC-P
Wang CY et al. Int J Med Sci 2021; 18 (1):256-269 Upregulation of peroxisome proliferator-activated receptor-α and the lipid metabolism pathway promotes carcinogenesis of ampullary cancer.
实验应用 : IHC-P
种属反应 : Human
Banerjee A et al. Sci Rep 2020; 10 (1):15443 Mechanistic study of attenuation of monosodium glutamate mixed high lipid diet induced systemic damage in rats by Coccinia grandis.
实验应用 : WB
Ling T et al. J Med Chem 2019; 62 (15):6925-6940 Mechanistic Insight on the Mode of Action of Colletoic Acid.
Guo S et al. Brain Res 2019; 1722:146353 Effects of angiotensin-II on brain endothelial cell permeability via PPARalpha regulation of para- and trans-cellular pathways.
实验应用 : WB
种属反应 : Human
Wang H et al. Am J Physiol Endocrinol Metab 2019; 316 (1):E54-E62 Adipose tissue transplantation ameliorates lipodystrophy-associated metabolic disorders in seipin-deficient mice.
实验应用 : WB
Orkhon B et al. Mol Med Rep 2018; (Epub) Astragalus root induces ovarian β-oxidation and suppresses estrogen-dependent uterine proliferation.
Kubo H et al. Lipids Health Dis 2017; 16 (1):106 Sake lees extract improves hepatic lipid accumulation in high fat diet-fed mice.
Song L et al. Sci Rep 2017; 7:41604 Phytosterol esters attenuate hepatic steatosis in rats with non-alcoholic fatty liver disease rats fed a high-fat diet.
Chung IH et al. Oncotarget 2016; 7 (16):22448-59 ChIP-on-chip analysis of thyroid hormone-regulated genes and their physiological significance.
实验应用 : WB
种属反应 : Human
Ye JH et al. Sci Rep 2016; 6:33102 Pentoxifylline ameliorates non-alcoholic fatty liver disease in hyperglycaemic and dyslipidaemic mice by upregulating fatty acid β-oxidation.
实验应用 : WB
Xu P et al. Nutr Metab (Lond) 2015; 12:58 diet rich in docosahexaenoic acid/eicosapentaenoic acid robustly ameliorates hepatic steatosis and * resistance in seipin deficient lipodystrophy mice.
实验应用 : WB
Hsu CY et al. Biogerontology 2014; Energy-regulated molecules maintain young status in the trophocytes and fat cells of old queen honeybees.
实验应用 : WB
种属反应 : Queen Bee
Lai YS et al. J Agric Food Chem 2014; Garlic essential oil protects against obesity-triggered nonalcoholic fatty liver disease through modulation of lipid metabolism and oxidative stress.
实验应用 : WB
Hsu CY et al. J Gerontol A Biol Sci Med Sci 2013; Changes in Energy-Regulated Molecules in the Trophocytes and Fat Cells of Young and Old Worker Honeybees (Apis mellifera).
实验应用 : WB
种属反应 : Honeybee
Ho FM et al. J Ethnopharmacol 2012; 142 (1):175-87 Anti-atherosclerotic action of Ger-Gen-Chyn-Lian-Tang and AMPK-dependent lipid lowering effect in hepatocytes.
实验应用 : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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