产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:2000 |
| 1:100-1:500 |
| 1:5000-1:10000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
0.01M TBS, 1% BSA, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
KLH conjugated synthetic phosphopeptide derived from human PPAR alpha around the phosphorylation site of ser12.
纯化方式
Protein A purified
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
peroxisome proliferator activated receptor alpha , NR1C1 , PPAR , PPARalpha , hPPAR
细胞定位
Nucleus
功能与背景
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX03713 WB Image
WB analysis of various sample lysates using GTX03713 PPAR alpha (phospho Ser12) antibody.
Lane 1 : Mouse Heart
Lane 2 : Mouse Liver
Loading : 40μg
Dilution : 1:300
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GTX03713 WB Image
WB analysis of various sample lysates using GTX03713 PPAR alpha (phospho Ser12) antibody.
Lane 1: Mouse Heart lysates
Lane 2 : Mouse Liver lysates
Lane 3 : Mouse Kidney lysates
Lane 4 : Mouse Cerebrum lysates
Lane 5 : Rat Cerebrum lysates
Lane 6 : Jurkat cell lysates
Lane 7 : A431 cell lysates
Dilution : 1:1000
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GTX03713 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX03713 PPAR alpha (phospho Ser12) antibody.
Dilution : 1:200
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GTX03713 ELISA Image
ELISA analysis of phosphopeptide and non-phosphorylated antigen using serial diluted GTX03713 PPAR alpha (phospho Ser12) antibody.
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GTX03713 WB Image
WB analysis of rat liver tissue lysate using GTX03713 PPAR alpha (phospho Ser12) antibody.
Loading : 40μg
Dilution : 1:300
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