产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Mouse brain , Molt-4 , HeLa , 293T , PPP3CB-transfected 293T
预测种属反应
Rat, Zebrafish, Rabbit, Bovine, Chicken, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human PPP3CB. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1951361
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
protein phosphatase 3 catalytic subunit beta , CALNA2 , CALNB , CNA2 , PP2Bbeta
功能与背景
Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.
数据库
研究领域
產品图片
![Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141](/templates/default/js/lightbox/images/loading.gif "Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141")
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GTX111141 WB Image
Wild-type (WT) and TLE1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PPP3CB antibody [N1N3] (GTX111141) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX111141 WB Image
Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX111141 diluted at 1:5000
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GTX111141 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111141 diluted at 1:1000
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GTX111141 IHC-P Image
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using PPP3CB(GTX111141) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX111141 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP3CB antibody [N1N3] (GTX111141) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
目前没有使用 PPP3CB antibody [N1N3] (GTX111141) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
目前没有使用 PPP3CB antibody [N1N3] (GTX111141) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141](/upload/website/prouct_img/small/GTX111141/GTX111141_40058_20170622_WB_KO_watermark_w_23060500_789.webp "Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141")
![Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141](/upload/website/prouct_img/small/GTX111141/GTX111141_WB_w_23060500_210.webp "Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141")
![Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141](/upload/website/prouct_img/small/GTX111141/GTX111141_40058_WB_M_Brain_w_23060500_351.webp "Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141")
![Anti-PPP3CB antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111141](/upload/website/prouct_img/small/GTX111141/GTX111141_40058_IHC_w_23060500_271.webp "Anti-PPP3CB antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111141")
![Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141](/upload/website/prouct_img/small/GTX111141/GTX111141_40058_20181109_WB_B_w_23060500_955.webp "Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141")
![Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141](/upload/website/prouct_img/normal/GTX111141/GTX111141_40058_20170622_WB_KO_watermark_w_23060500_789.webp "Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141")
![Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141](/upload/website/prouct_img/normal/GTX111141/GTX111141_WB_w_23060500_210.webp "Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141")
![Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141](/upload/website/prouct_img/normal/GTX111141/GTX111141_40058_WB_M_Brain_w_23060500_351.webp "Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141")
![Anti-PPP3CB antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111141](/upload/website/prouct_img/normal/GTX111141/GTX111141_40058_IHC_w_23060500_271.webp "Anti-PPP3CB antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111141")
![Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141](/upload/website/prouct_img/normal/GTX111141/GTX111141_40058_20181109_WB_B_w_23060500_955.webp "Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141")
