Non-infected (–) and infected (+) Marc-145 whole cell extracts were separated by 15% SDS-PAGE, and the membrane was blotted with PRRS virus GP5 protein antibody (GTX129062) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) Marc-145 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PRRS virus GP5 protein antibody (GTX129062) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.