产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:2,000 |
| 1:250 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody detects ~42, 46, 48, and 52 kDa proteins which correspond to subunit 10B and probably the combined signal from 4, 6B, 7, and 8 subunit proteins.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
Batch dependent (Please refer to the vial label for the specific concentration.)
免疫原
Synthetic Peptide: C A(278) T N R P D T L D P A L L R P G R(294)
纯化方式
Ammonium sulfate precipitation
偶联
Unconjugated
RRID
AB_11174017
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
proteasome 26S subunit, non-ATPase 2 , P97 , RPN1 , S2 , TRAP2
功能与背景
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
数据库
研究领域
產品图片
|
GTX79499 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX79499 PSMD2 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature
|
|
|
GTX79499 WB Image
WB analysis of MCF7 (Lane 1), PC-3 (Lane 2), Hep G2 (Lane 3), SK-OV-3 (Lane 4), HeLa (Lane 5), COS-7 (Lane 6), Jurkat (Lane 7), A-431 (Lane 8), tissue extracts of mouse testis (Lane 9) and rat testis (Lane 10) using GTX79499 PSMD2 antibody.
Dilution : 1:1000
|
文献引用
目前没有使用 PSMD2 antibody (GTX79499) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
目前没有使用 PSMD2 antibody (GTX79499) 的产品评论被收录。快发表第一篇产品评论吧！
