产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
human PPY-transfected 293T cells
预测种属反应
Bovine, Dog, Guinea pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Pancreatic polypeptide. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888058
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
pancreatic polypeptide , PNP , PP
细胞定位
Secreted
功能与背景
This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded 95 aa preproprotein is synthesized in the pancreatic islets of Langerhans and proteolytically processed to generate two peptide products. These products include the active pancreatic hormone of 36 aa and an icosapeptide of unknown function. This hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
数据库
研究领域
產品图片
![Anti-Pancreatic Polypeptide antibody [GT6512] used in IHC (Paraffin sections) (IHC-P). GTX628832](/templates/default/js/lightbox/images/loading.gif "Anti-Pancreatic Polypeptide antibody [GT6512] used in IHC (Paraffin sections) (IHC-P). GTX628832")
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GTX628832 IHC-P Image
Pancreatic Polypeptide antibody [GT6512] detects Pancreatic Polypeptide protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse pancreas.Pancreatic Polypeptide stained by Pancreatic Polypeptide antibody [GT6512] (GTX628832) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX628832 WB Image
Pancreatic polypeptide antibody [GT6512] detects PPY protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug whole cell lysate/extract of human PPY-transfected 293T cells
15 % SDS-PAGE
Pancreatic polypeptide antibody [GT6512] (GTX628832) dilution: 1:5000
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GTX628832 ICC/IF Image
Pancreatic Polypeptide antibody [GT6512] detects Pancreatic Polypeptide protein at cytoplasm by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pancreatic Polypeptide stained by Pancreatic Polypeptide antibody [GT6512] (GTX628832) diluted at 1:2000.Blue: Hoechst 33342 staining.Scale bar= 10μm.
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文献引用
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产品评论
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![Anti-Pancreatic Polypeptide antibody [GT6512] used in IHC (Paraffin sections) (IHC-P). GTX628832](/upload/website/prouct_img/small/GTX628832/GTX628832_41267_20180629_IHC-P_M_w_23061202_457.webp "Anti-Pancreatic Polypeptide antibody [GT6512] used in IHC (Paraffin sections) (IHC-P). GTX628832")
![Anti-Pancreatic Polypeptide antibody [GT6512] used in Western Blot (WB). GTX628832](/upload/website/prouct_img/small/GTX628832/GTX628832_41267_WB_B_w_23061202_666.webp "Anti-Pancreatic Polypeptide antibody [GT6512] used in Western Blot (WB). GTX628832")
![Anti-Pancreatic Polypeptide antibody [GT6512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628832](/upload/website/prouct_img/small/GTX628832/GTX628832_41267_20180725_ICC_IF_B_w_23061202_888.webp "Anti-Pancreatic Polypeptide antibody [GT6512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628832")
![Anti-Pancreatic Polypeptide antibody [GT6512] used in IHC (Paraffin sections) (IHC-P). GTX628832](/upload/website/prouct_img/normal/GTX628832/GTX628832_41267_20180629_IHC-P_M_w_23061202_457.webp "Anti-Pancreatic Polypeptide antibody [GT6512] used in IHC (Paraffin sections) (IHC-P). GTX628832")
![Anti-Pancreatic Polypeptide antibody [GT6512] used in Western Blot (WB). GTX628832](/upload/website/prouct_img/normal/GTX628832/GTX628832_41267_WB_B_w_23061202_666.webp "Anti-Pancreatic Polypeptide antibody [GT6512] used in Western Blot (WB). GTX628832")
![Anti-Pancreatic Polypeptide antibody [GT6512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628832](/upload/website/prouct_img/normal/GTX628832/GTX628832_41267_20180725_ICC_IF_B_w_23061202_888.webp "Anti-Pancreatic Polypeptide antibody [GT6512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628832")
