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Pax2a抗体

Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127
Anti-Pax2a antibody used in Western Blot (WB). GTX128127
Anti-Pax2a antibody used in Western Blot (WB). GTX128127
Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127
Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127
Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127

目录号 GTX128127

文献引用 15

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-Wm

种属反应

 Zebrafish, Japanese Medaka
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 zebrafish Pax2a 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-Wm 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

44 kDa. ( Note )

阳性对照

2dpf zebrafish embryos , 36 hpf zebrafish embryos

预测种属反应

Human, Mouse, Sheep, Chicken(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.21 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

Recombinant protein encompassing a sequence within the center region of zebrafish Pax2a. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2630322

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

paired box 2a , PAXZF-B , Pax-2 , cb378 , noi , pax-b , pax2.1 , pax2a1 , pax[zf-b] , paxb

细胞定位

Nucleus

功能与背景

Probable transcription factor involved in the development of the midbrain/hindbrain boundary (MHB) organizer and specification of neuronal cell fates. Required for establishment of eng2 and eng3 gene expression in the midbrain and MHB primordium during late gastrulation.

数据库

研究领域

產品图片

Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127

GTX128127 IHC-Wm Image

Pax2a antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Green: Pax2a antibody (GTX128127) dilution: 1:200.
Red: Tubulin (Acetyl Lys40) antibody [6-11B-1] (GTX16292) dilution: 1:50.
Anti-Pax2a antibody used in Western Blot (WB). GTX128127

GTX128127 WB Image

Zebrafish tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Pax2a antibody (GTX128127) diluted at 1:1000.

Anti-Pax2a antibody used in Western Blot (WB). GTX128127

GTX128127 WB Image

Zebrafish embryos extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Pax2a antibody (GTX128127) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127

GTX128127 IHC-Wm Image

Pax2a antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Green: Pax2a antibody (GTX128127) dilution: 1:200.
Red: Tubulin (Acetyl Lys40) antibody [6-11B-1] (GTX16292) dilution: 1:50.
Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127

GTX128127 IHC-Wm Image

Pax2a antibody detects Pax2a protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Pax2a antibody (GTX128127) dilution: 1:100.
Anti-Pax2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128127

GTX128127 IHC-Wm Image

The data was published in the journal Sci Rep in 2018.PMID: 29618800

文献引用

Application Reference

EA Perens et al. bioRxiv 2024; Drivers of Vessel Progenitor Fate Define Intermediate Mesoderm Dimensions by Inhibiting Kidney Progenitor Specification
实验应用 : IHC-Wm
Haerlingen B et al. Development 2023; 150 (10) Mesodermal FGF and BMP govern the sequential stages of zebrafish thyroid specification.
Macaulie A Casey et al. G3 (Bethesda) 2022; 12 (3) Shutdown corner, a large deletion mutant isolated from a haploid mutagenesis screen in zebrafish.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Karin D Prummel et al. Nat Commun 2022; 13 (1) : 1677 Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma.
实验应用 : IHC-Wm
Nandamuri SP et al. PLoS One 2022; 17 (3) : e0265327 Loss of zebrafish dzip1 results in inappropriate recruitment of periocular mesenchyme to the optic fissure and ocular coloboma.
实验应用 : IHC-Wm
种属反应 : Zebrafish
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Application Reference
EA Perens et al. bioRxiv 2024; Drivers of Vessel Progenitor Fate Define Intermediate Mesoderm Dimensions by Inhibiting Kidney Progenitor Specification
实验应用 : IHC-Wm
Haerlingen B et al. Development 2023; 150 (10) Mesodermal FGF and BMP govern the sequential stages of zebrafish thyroid specification.
Macaulie A Casey et al. G3 (Bethesda) 2022; 12 (3) Shutdown corner, a large deletion mutant isolated from a haploid mutagenesis screen in zebrafish.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Karin D Prummel et al. Nat Commun 2022; 13 (1):1677 Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma.
实验应用 : IHC-Wm
Nandamuri SP et al. PLoS One 2022; 17 (3):e0265327 Loss of zebrafish dzip1 results in inappropriate recruitment of periocular mesenchyme to the optic fissure and ocular coloboma.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Perens EA et al. Development 2021; 148 (15) osr1 couples intermediate mesoderm cell fate with temporal dynamics of vessel progenitor cell differentiation.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Branigan GL et al. J Dev Biol 2021; 9 (4) Zebrafish Paralogs brd2a and brd2b Are Needed for Proper Circulatory, Excretory and Central Nervous System Formation and Act as Genetic Antagonists during Development.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Gillotay P et al. EMBO Rep 2020; 21 (12):e50612 Single-cell transcriptome analysis reveals thyrocyte diversity in the zebrafish thyroid gland.
Bryan CD et al. Development 2020; 147 (4) Optic cup morphogenesis requires neural crest-mediated basement membrane assembly.
Deeptha Vasudevan et al. bioRxiv 2020; (Epub) Regenerated Interneurons Integrate Into Locomotor Circuitry Following Spinal Cord Injury.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Gordon HB et al. Development 2018; 145 (22) Hedgehog signaling regulates cell motility and optic fissure and stalk formation during vertebrate eye morphogenesis.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Chase Dallas Bryan et al. bioRxiv 2018; (Epub) Neural crest cells regulate optic cup morphogenesis by promoting extracellular matrix assembly.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Trubiroha A et al. Sci Rep 2018; 8 (1):5647 A Rapid CRISPR/Cas-based Mutagenesis Assay in Zebrafish for Identification of Genes Involved in Thyroid Morphogenesis and Function.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Sedykh I et al. Dev Biol 2017; Zebrafish zic2 controls formation of periocular neural crest and choroid fissure morphogenesis.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Perens EA et al. Elife 2016; 5 (Epub) Hand2 inhibits kidney specification while promoting vein formation within the posterior mesoderm.
实验应用 : IHC-Wm
种属反应 : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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