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Paxillin抗体

KO/KD Overexpression
Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Immunoprecipitation (IP). GTX125891
Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891
Anti-Paxillin antibody used in Western Blot (WB). GTX125891
Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891

目录号 GTX125891

文献引用 11
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Paxillin 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

65 kDa. ( Note )

阳性对照

GFP-human Paxillin-transfected 293T cells , rat brain , HeLa

预测种属反应

Chicken(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Paxillin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2885610

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

paxillin

细胞定位

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

数据库

研究领域

產品图片

Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891

GTX125891 ICC/IF Image

Paxillin antibody detects Paxillin protein at cytoskeleton by immunofluorescent analysis.
Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin protein stained by Paxillin antibody (GTX125891) diluted at 1:100.
Red: phalloidin staining.
Courtesy of a researcher who prefers to be anonymous.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

KO/KD

Wild-type (WT) and Paxillin knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Immunoprecipitation (IP). GTX125891

GTX125891 IP Image

Paxillin antibody immunoprecipitates Paxillin protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 2 μg of preimmune Rabbit IgG
B. Immunoprecipitation of Paxillin protein by 2 μg Paxillin antibody (GTX125891)
7.5 % SDS-PAGE
The immunoprecipitated Paxillin protein was detected by Paxillin antibody (GTX125891) diluted at 1:10000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891

GTX125891 IHC-P Image

Paxillin antibody detects Paxillin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Paxillin stained by Paxillin antibody (GTX125891) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Overexpression

Paxillin antibody detects Paxillin protein by western blot analysis.
A. 1 μg 293T whole cell extract
B. 1 μg whole cell extract of GFP-human Paxillin-transfected 293T cells
7.5% SDS-PAGE
Paxillin antibody (GTX125891) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in IHC (Paraffin sections) (IHC-P). GTX125891

GTX125891 IHC-P Image

Paxillin antibody detects Paxillin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Paxillin stained by Paxillin antibody (GTX125891) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Paxillin antibody used in Western Blot (WB). GTX125891

GTX125891 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody (GTX125891) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891

GTX125891 ICC/IF Image

The data was published in the journal J Cell Biol in 2020.PMID: 31816055

文献引用

Application Reference

Ramella M et al. iScience 2024; 27 (4) : 109440 Dual specificity kinase DYRK3 regulates cell migration by influencing the stability of protrusions.
Kumari R et al. Nat Commun 2024; 15 (1) : 2547 Focal adhesions contain three specialized actin nanoscale layers.
Ripamonti M et al. Commun Biol 2022; 5 (1) : 1025 A functional interaction between liprin-α1 and B56γ regulatory subunit of protein phosphatase 2A supports tumor cell motility.
Yin-Quan Chen et al. Front Cell Dev Biol 2022; 10 : 809738 Snail Augments Nuclear Deformability to Promote Lymph Node Metastasis of Head and Neck Squamous Cell Carcinoma.
实验应用 : ICC/IF
种属反应 : Human
Schiweck J et al. Nat Commun 2021; 12 (1) : 1490 Drebrin controls scar formation and astrocyte reactivity upon traumatic brain injury by regulating membrane trafficking.
实验应用 : ICC/IF
种属反应 : Mouse
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产品评论

Paxillin antibody 目录号 GTX125891

Rating
( Average 5 based on 2 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 2 users reviews)
Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891
Date : Anonymous submitted on 30-Mar-2016
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Y1 cells
Blocking : Commercial Blocking buffer, 25°C, 1Hr
Fixation : paraformaldehyde, 25°C, 0.3Hr
Permeabilization /Antigen retrieval : 0.5% Triton X-100, 25°C, 10minHr
Primary Antibody : 1:500, 4°C, 16Hr
Anti-Paxillin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125891
Date : Anonymous submitted on 20-Mar-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : MDA-MB-231 cells
Blocking : 3% BSA / 0.02% Triton-X100 in PBS, 25°C, 1Hr
Fixation : 4% paraformaldehyde
Primary Antibody : 1:100, 4°C, 16Hr
我要评论产品
Application Reference
Ramella M et al. iScience 2024; 27 (4):109440 Dual specificity kinase DYRK3 regulates cell migration by influencing the stability of protrusions.
Kumari R et al. Nat Commun 2024; 15 (1):2547 Focal adhesions contain three specialized actin nanoscale layers.
Ripamonti M et al. Commun Biol 2022; 5 (1):1025 A functional interaction between liprin-α1 and B56γ regulatory subunit of protein phosphatase 2A supports tumor cell motility.
Yin-Quan Chen et al. Front Cell Dev Biol 2022; 10:809738 Snail Augments Nuclear Deformability to Promote Lymph Node Metastasis of Head and Neck Squamous Cell Carcinoma.
实验应用 : ICC/IF
种属反应 : Human
Schiweck J et al. Nat Commun 2021; 12 (1):1490 Drebrin controls scar formation and astrocyte reactivity upon traumatic brain injury by regulating membrane trafficking.
实验应用 : ICC/IF
种属反应 : Mouse
Lin JW et al. Cell Death Discov 2021; 7 (1):35 Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals.
实验应用 : WB
种属反应 : Mouse
Masi I et al. Cell Rep 2021; 34 (9):108800 Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK.
实验应用 : WB
种属反应 : Human
Atherton P et al. J Cell Biol 2020; 219 (1) Relief of talin autoinhibition triggers a force-independent association with vinculin.
Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.
实验应用 : WB
种属反应 : Human
Megan R. Chastney et al. bioRxiv 2020; (Epub) Brain insulin resistance triggers early onset Alzheimer disease in Down syndrome.
实验应用 : ICC/IF
种属反应 : Mouse
Borgo C et al. Sci Rep 2017; 7:42409 Generation and quantitative proteomics analysis of CK2α/α'(-/-) cells.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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