产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
A431 (1 mM pervanadate , 37ºC treatment for 30 min) , HeLa
预测种属反应
Mouse, Rat, Chicken, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr118 of human Paxillin. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2888361
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
paxillin
细胞定位
Cytoplasm , cytoskeleton , Cell junction , focal adhesion
数据库
研究领域
產品图片
![Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678](/templates/default/js/lightbox/images/loading.gif "Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678")
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GTX633678 WB Image
Untreated (–) and treated (+) A431 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:3000.
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GTX633678 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:500.
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GTX633678 ICC/IF Image
Paxillin (phospho Tyr118) antibody [GT162] detects Paxillin (phospho Tyr118) protein at focal adhesion sites by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin (phospho Tyr118) protein stained by Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:200.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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文献引用
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产品评论
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![Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678](/upload/website/prouct_img/small/GTX633678/GTX633678_42604_20170105_WB_Pervanadate_w_23061202_821.webp "Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678")
![Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678](/upload/website/prouct_img/small/GTX633678/GTX633678_42604_20170119_WB_shRNA_watermark_w_23061202_202.webp "Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678")
![Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633678](/upload/website/prouct_img/small/GTX633678/GTX633678_42604_20161206_IFA_w_23061202_613.webp "Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633678")
![Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678](/upload/website/prouct_img/normal/GTX633678/GTX633678_42604_20170105_WB_Pervanadate_w_23061202_821.webp "Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678")
![Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678](/upload/website/prouct_img/normal/GTX633678/GTX633678_42604_20170119_WB_shRNA_watermark_w_23061202_202.webp "Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Western Blot (WB). GTX633678")
![Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633678](/upload/website/prouct_img/normal/GTX633678/GTX633678_42604_20161206_IFA_w_23061202_613.webp "Anti-Paxillin (phospho Tyr118) antibody [GT162] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633678")
