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Paxillin (phospho Tyr118)抗体 [GT712]

KO/KD Orthogonal
Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633
Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633
Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633
Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633633
Anti-Paxillin (phospho Tyr118) antibody [GT712] used in IHC (Paraffin sections) (IHC-P). GTX633633

目录号 GTX633633

1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT712

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

65 kDa. ( Note )

阳性对照

Rat2

预测种属反应

Chicken, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr118 of human Paxillin. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888357

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

paxillin

细胞定位

Cytoplasm , cytoskeleton , Cell junction , focal adhesion

数据库

研究领域

產品图片

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633

GTX633633 WB Image

KO/KD

Wild-type (WT) and Paxillin (phospho Tyr118) knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633

GTX633633 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633

GTX633633 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633633

GTX633633 ICC/IF Image

Paxillin (phospho Tyr118) antibody [GT712] detects Paxillin (phospho Tyr118) protein at focal adhesion by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Paxillin (phospho Tyr118) stained by Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Paxillin (phospho Tyr118) antibody [GT712] used in IHC (Paraffin sections) (IHC-P). GTX633633

GTX633633 IHC-P Image

Paxillin (phospho Tyr118) antibody [GT712] detects Paxillin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Paxillin stained by Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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Paxillin (phospho Tyr118) antibody [GT712] 目录号 GTX633633

Rating
( Average 5 based on 1 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633633
Date : Anonymous submitted on 21-Sep-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : ARPE-19 cells
Blocking : 3% BSA and 0.02% Triton-X100 in PBS, 25°C, 1Hr
Primary Antibody : 1:50, 4°C, 16Hr
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SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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