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Pcna抗体

Anti-Pcna antibody used in Western Blot (WB). GTX124496
Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496
Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496
Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496
Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496
Anti-Pcna antibody used in IHC (Paraffin sections) (IHC-P). GTX124496
Anti-Pcna antibody used in Western Blot (WB). GTX124496
Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496
Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496
Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496
Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

目录号 GTX124496

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, IHC-P, IHC-Fr, IHC-Wm

种属反应

Zebrafish, Japanese Medaka, Nothobranchius furzeri
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:50-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

29 kDa. ( Note )

阳性对照

zebrafish heart , zebrafish muscle , whole zebrafish , zebrafish muscle

预测种属反应

Human (100%)| Mouse (97%)| Rat (98%)| Zebrafish (92%)| Bovine (99%)| Chicken (96%)| Pig (100%)| Xenopus tropicalis (89%)| Rhesus Monkey (100%)| Killifish (92%)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

The immunogen used to generate this antibody corresponds to zebrafish Pcna

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11161916

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

proliferating cell nuclear antigen , cb16 , fa28e03 , fb36g03 , wu:fa28e03 , wu:fb36g03

细胞定位

Nucleus

功能与背景

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.

数据库

研究领域

產品图片

Anti-Pcna antibody used in Western Blot (WB). GTX124496

GTX124496 WB Image

Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Pcna antibody (GTX124496) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

GTX124496 IHC-Wm Image

Pcna antibody detects Pcna protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Pcna antibody (GTX124496) dilution: 1:50.

Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

GTX124496 IHC-Wm Image

Pcna antibody detects Pcna protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Pcna antibody (GTX124496) dilution: 1:50.

Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

GTX124496 IHC-Wm Image

Pcna antibody detects Pcna protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Pcna antibody (GTX124496) dilution: 1:100.

Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

GTX124496 IHC-Wm Image

Pcna antibody detects Pcna protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Pcna antibody (GTX124496) dilution: 1:100.

Anti-Pcna antibody used in IHC (Paraffin sections) (IHC-P). GTX124496

GTX124496 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish kidney, using pcna (GTX124496) antibody at 1:300 dilution.

Anti-Pcna antibody used in Western Blot (WB). GTX124496

GTX124496 WB Image

The data was published in the journal Cells in 2019.PMID: 31569376

Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

GTX124496 IHC-Wm Image

The data was published in the 2019 in Front Mol Neurosci. PMID: 31616250

Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

GTX124496 IHC-Wm Image

The data was published in the journal Development in 2018.PMID: 29695612

Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

GTX124496 IHC-Wm Image

The data was published in the journal Development in 2018.PMID: 29695612

Anti-Pcna antibody used in IHC (Whole mount) (IHC-Wm). GTX124496

GTX124496 IHC-Wm Image

The data was published in the journal Development in 2018.PMID: 29695612

文献引用

产品评论

Pcna antibody 目录号 GTX124496

Rating ( Average 4 based on 1 users reviews)
实验应用
IHC (Frozen sections)(IHC-Fr) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 03-Aug-2018
Score :
Application Tested : IHC-Fr
Sample Species : Zfsh
Sample : 3 days post amputation(dpa) zebrafish caudal fin blastema
Blocking : 1.5% skim milk, 25°C, 0.5Hr
Fixation : 4% PFA/ PBS
Permeabilization /Antigen retrieval : 10 mM Citrate buffer, 105°C, 1 minHr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Red: Pcna stained by Pcna antibody (GTX124496) diluted at 1:500. Blue: DAPI
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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