产品说明
摘要
Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| Assay dependent |
|
Note :
Conjugation
Azide should be removed.
Purification
Conjugation to Sepharose 4B (solid phase columns) to purify mucin type glycoproteins. Inhibiting/Eluting sugars : 200 mM Galactose
|
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
产品说明
Carbohydrate-Binding Specificity of Peanut Lectin : Galβ1, 3 GalNAc>>GalNAc>Gal
产品属性
形式
Liquid
存储溶液
10mM Bicarbonate, 150mM NaCl, 0.1mM Calcium Chloride (pH8.2). Dilute in 0.1mM Calcium Chloride buffer.
保存剂
0.05% Sodium azide
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
浓度
5 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
Native Protein
表达系统
Native Protein
纯化方式
Purified from Peanut
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
PNA lectin , PNA , Arachis hypogaea , Peanut Lectin , Peanut glutinin ctin , Arachis hypogaea , Peanut agglutinin , Arachis hypogaea lectin , Peanut agglutinin lectin
功能与背景
Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit.
Inhibiting/Eluting sugars: 200 mM GalactoseCarbohydrate-Binding Specificity of PNA: Galβ1, 3 GalNAc>>>>>GalNAc>Gal
研究领域
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