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Antigen retrieval condition-Pepsin (ready to use)

目录号 GTX28194

实验应用

IHC-P
产品包装
50 ml (¥5200)
产品说明

摘要

Antigen retrieval solution-enzymatic (pepsin)

产品应用

应用说明

This antigen retrieval solution is provided in a ready-to-use format requiring no further preparation. 1.) Apply this solution to tissue sections following their initial hydration step. Incubate for 10 min at 37ºC or 45ºC (this incubation time may vary depending on the degree of fixation of the tissue sections. 2.) Wash tissue sections well with IHC wash buffer (GTX28195), or PBS or TBS if immunostaining by hand.

产品属性

形式

Liquid

存放说明

Refridgerate at 4ºC. DO NOT FREEZE. Avoid extended exposure to room temperature.

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

功能与背景

Most fixatives immobilize the tissue antigens by cross linkages. Sometimes, extensive cross linking of proteins in high concentrations can also lead to the masking or destruction of antigens, which leads to poor staining or no staining at all. This loss of staining can be restored to a certain extent by pretreatment of tissue sections with proteolytic enzymes. The unmasking of tissue sections is an important requirement for the optimal staining of antigens like immunoglobulins, keratins, carcinoembryonic antigen, factor VIII related antigen, testosterone, and to a lesser extent S100 protein.

研究领域

文献引用

产品评论

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产品包装 报价 (¥)
¥ 5200
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