Mixing of reagent: To five ml buffer (reagent B) in a test tube, add one drop of concentrated Enhancing reagent (reagent E) mix well. This ready to use reagent is good for 4-6 hours. Procedure: 1.) After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated) and distilled/ deionized water. 2.) Add few drops of ready to use enhancing reagent for 1-2 minutes. 3.) Add few drops of ready to use AEC reagent, or DAB reagent, follow instructions as outlined for these chromogens.
