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Porcine circovirus type 2 / PCV2 Capsid抗体

Anti-Porcine circovirus type 2 / PCV2 Capsid antibody used in IHC (Paraffin sections) (IHC-P). GTX128121
Anti-Porcine circovirus type 2 / PCV2 Capsid antibody used in IHC (Paraffin sections) (IHC-P). GTX128121
Anti-Porcine circovirus type 2 / PCV2 Capsid antibody used in Western Blot (WB). GTX128121
Anti-Porcine circovirus type 2 / PCV2 Capsid antibody used in Western Blot (WB). GTX128121

目录号 GTX128121

文献引用 5

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P, FISH

种属反应

 Porcine circovirus type 2
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Porcine circovirus type 2 Capsid 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
FISH Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

28 kDa. ( Note )

阳性对照

PCV2-infected PK-15 , PCV2 transfected

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Porcine circovirus type 2

免疫原

Recombinant fragment of Porcine circovirus type 2 Capsid protein. (Strain: swPCV2bps1)

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2885711

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Porcine circovirus Capsid , PCV Capsid

功能与背景

The PCV2 virion measures approximately 17 nm in diameter, is non-enveloped and consists of a circular single-stranded DNA surrounded by an icosahedral capsid. The ambisense DNA molecule contains about 1.77 kb and 11 putative open reading frames (ORFs).
PCV2 has been associated with a number of disease syndromes in pigs. Post-weaning multisystemic wasting syndrome (PMWS) is an important emerging viral disease affecting nursery and grower pigs, which has recently been associated with porcine circovirus type 2 (PCV2) infections. PMWS has been reported in Europe, South East Asia, and the Americas.

研究领域

產品图片

Anti-Porcine circovirus type 2 / PCV2 Capsid antibody used in IHC (Paraffin sections) (IHC-P). GTX128121

GTX128121 IHC-P Image

Porcine circovirus type 2 Capsid antibody detects Porcine circovirus type 2 Capsid protein by immunohistochemical analysis.
Sample: Paraffin-embedded swine lymph node.
Porcine circovirus type 2 Capsid stained by Porcine circovirus type 2 Capsid antibody (GTX128121) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Porcine circovirus type 2 / PCV2 Capsid antibody used in IHC (Paraffin sections) (IHC-P). GTX128121

GTX128121 IHC-P Image

Porcine circovirus type 2 Capsid antibody detects Porcine circovirus type 2 Capsid protein by immunohistochemical analysis.
Sample: Paraffin-embedded swine spleen.
Porcine circovirus type 2 Capsid stained by Porcine circovirus type 2 Capsid antibody (GTX128121) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Porcine circovirus type 2 / PCV2 Capsid antibody used in Western Blot (WB). GTX128121

GTX128121 WB Image

Infected (+) PK-15 whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with Porcine circovirus type 2 Capsid antibody (GTX128121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Porcine circovirus type 2 / PCV2 Capsid antibody used in Western Blot (WB). GTX128121

GTX128121 WB Image

Lysate (30 μg) from Sf21 cells, expressing recombinant PCV2 protein by baculovirus system, was separated by 12% SDS-PAGE, and the membrane was blotted with Porcine circovirus type 2 Capsid antibody (GTX128121) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The lysate was provided courtesy of Dr. Wu lab at Chung Yuan Christian University, Department of Bioscience Technology.)
Infected Sf21 whole cell extract provided from Dr. Wu lab, Chung Yuan Christian University, Department of Bioscience Technology.

文献引用

Application Reference

Tao Y et al. Nat Commun 2023; 14 (1) : 443 Highly efficient and robust π-FISH rainbow for multiplexed in situ detection of diverse biomolecules.
实验应用 : FISH
Hosono S et al. J Virol Methods 2019; 273 : 113706 Porcine circovirus type 2 induces a strong cytopathic effect in the serum-free culture cell line CPK-NS.
Tsai GT et al. Sci Rep 2019; 9 (1) : 10782 Phylogeographic and genetic characterization of porcine circovirus type 2 in Taiwan from 2001-2017.
实验应用 : IHC-P
Ouyang T et al. Viruses 2019; 11 (6) Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins.
实验应用 : WB
Liu X et al. Sci Rep 2019; 9 (1) : 5638 Human cells are permissive for the productive infection of porcine circovirus type 2 in vitro.
实验应用 : WB
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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