产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Neuro2A , NIH-3T3 , C2C12 , IMR32
预测种属反应
Rat, Rabbit, Sheep, Bovine, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.36 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Prion Protein (PrP). The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10617796
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
prion protein , ASCR , AltPrP , CD230 , CJD , GSS , KURU , PRIP , PrP , PrP27-30 , PrP33-35C , PrPc , p27-30
细胞定位
Cell membrane; Lipid-anchor , GPI-anchor , Golgi apparatus
功能与背景
The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX101063 WB Image
Sample (30 ug of whole cell lysate)
A:IMR32
12% SDS PAGE
GTX101063 diluted at 1:1000
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GTX101063 WB Image
PRP antibody detects PRNP protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg C2C12 whole cell lysate/extract
12 % SDS-PAGE
PRP antibody (GTX101063) dilution: 1:1000
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GTX101063 IHC-P Image
PRP antibody detects PRP protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
PRP antibody (GTX101063) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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