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Prohibitin抗体

KO/KD Orthogonal
Anti-Prohibitin antibody used in Western Blot (WB). GTX101105
Anti-Prohibitin antibody used in Western Blot (WB). GTX101105
Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105
Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105
Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105
Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105
Anti-Prohibitin antibody used in Western Blot (WB). GTX101105
Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105
Anti-Prohibitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101105
Anti-Prohibitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101105
Anti-Prohibitin antibody used in Immunoprecipitation (IP). GTX101105
Anti-Prohibitin antibody used in Western Blot (WB). GTX101105
Anti-Prohibitin antibody used in Western Blot (WB). GTX101105
Anti-Prohibitin antibody used in Western Blot (WB). GTX101105

目录号 GTX101105

文献引用 6
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

30 kDa. ( Note )

阳性对照

NIH-3T3 , PC-12 , 293T

预测种属反应

Zebrafish, Bovine, Cat, Dog, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1951209

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

prohibitin , HEL-215 , HEL-S-54e , PHB1

细胞定位

Mitochondrion inner membrane

功能与背景

Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Prohibitin antibody used in Western Blot (WB). GTX101105

GTX101105 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Prohibitin antibody (GTX101105) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Prohibitin antibody used in Western Blot (WB). GTX101105

GTX101105 WB Image

Prohibitin antibody detects PHB protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
Prohibitin antibody (GTX101105) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105

GTX101105 IHC-P Image

Prohibitin antibody detects Prohibitin protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Prohibitin antibody (GTX101105) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105

GTX101105 IHC-P Image

Prohibitin antibody detects Prohibitin protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Prohibitin antibody (GTX101105) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105

GTX101105 IHC-P Image

Prohibitin antibody detects Prohibitin protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Prohibitin stained by Prohibitin antibody (GTX101105) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105

GTX101105 IHC-P Image

Prohibitin antibody detects Prohibitin protein at mitochondria in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Prohibitin antibody (GTX101105) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Prohibitin antibody used in Western Blot (WB). GTX101105

GTX101105 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
12% SDS PAGE
GTX101105 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105

GTX101105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Prohibitin(GTX101105) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Prohibitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101105

GTX101105 ICC/IF Image

Prohibitin antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Prohibitin protein stained by Prohibitin antibody (GTX101105) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Anti-Prohibitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101105

GTX101105 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Prohibitin(GTX101105) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Prohibitin antibody used in Immunoprecipitation (IP). GTX101105

GTX101105 IP Image

Immunoprecipitation of Prohibitin protein from 293T whole cell extracts using 5 μg of Prohibitin antibody (GTX101105).
Western blot analysis was performed using Prohibitin antibody (GTX101105).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Prohibitin antibody used in Western Blot (WB). GTX101105

GTX101105 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Prohibitin antibody (GTX101105) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Prohibitin antibody used in Western Blot (WB). GTX101105

GTX101105 WB Image

Orthogonal

The data was published in the journal J Biomed Sci in 2020.PMID: 32169072

Anti-Prohibitin antibody used in Western Blot (WB). GTX101105

GTX101105 WB Image

The data was published in the journal J Biomed Sci in 2020.PMID: 32169072

文献引用

Application Reference

Chiu YC et al. Biochem Biophys Rep 2021; 28 : 101166 Accumulation of cholesterol suppresses oxidative phosphorylation and altered responses to inflammatory stimuli of macrophages.
实验应用 : ICC/IF
种属反应 : Mouse
Fang CH et al. J Biomed Sci 2020; 27 (1) : 42 A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and 帣-catenin-ABCG2 signaling.
实验应用 : WB
种属反应 : Human
Heng-Hsiung Wu et al. Journal of Food and Drug Analysis 2019; (Epub) Involvement of prohibitin 1 and prohibitin 2 upregulation in cBSA-induced podocyte cytotoxicity.
实验应用 : IHC-P
种属反应 : Mouse
Moriggi M et al. Proteomics 2017; (Epub) Contribution of Extracellular Matrix and Signal Mechanotransduction to Epithelial Cell Damage in Inflammatory Bowel Disease Patients: A Proteomic Study.
实验应用 : WB
种属反应 : Human
Yurugi H et al. Oncogene 2017; (Epub) Targeting prohibitins with chemical ligands inhibits KRAS-mediated lung tumours.
实验应用 : WB
种属反应 : Human
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产品评论

Prohibitin antibody 目录号 GTX101105

Rating
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实验应用
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-Prohibitin antibody used in IHC (Paraffin sections) (IHC-P). GTX101105
Date : Anonymous submitted on 10-May-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Kidney tissue
Fixation : Paraffin-embedded
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.5Hr
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Mouse kidney, 16 week.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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