产品说明
摘要
Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of Protein G. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm, Fluorophore/Protein: A492nm/A280nm
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
产品属性
形式
Liquid
存储溶液
10mM Phosphate, 150mM NaCl, 1% BSA
保存剂
0.05% Sodium azide
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
Recombinant Protein G (FITC)
表达系统
E. coli
偶联
Fluorescein isothiocyanate (FITC)
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
功能与背景
Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of Protein G. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm, Fluorophore/Protein: A492nm/A280nm
研究领域
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