Dot blot analysis of various samples using GTX27249 Protein G antibody (FITC).
Row 1 : Human IgG
Row 2 : Rat IgG
Row 3 : Dog IgG
Dilution : 1:1000

Dot Blot of Protein G Antibody Fluorescein Conjugated (GTX27249). Antigen 1: Human IgG. Antigen 2: Rat IgG. Antigen 3: Dog IgG. Load: Starting dot is 200 ng followed by a 3-fold serial dilution. Primary antibody: Protein G at 1:1000 for 60 min RT. Secondary antibody: Fluorescein rabbit anti-Protein G at 1:1,000 for 30 min at RT. Block: blocking buffer for 30 min at RT.