Pyruvate Kinase (liver/RBC)抗体

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein by Western blot analysis.
A. 20 ug Rat liver lysate/extract
7.5% SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (GTX101033) dilution: 1:10000

Sample (30 ug of whole cell lysate) 
  A: Jurkat
  B: Raji
  C: K562
  7.5% SDS PAGE 
  GTX101033 diluted at 1:1000

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pyruvate Kinase (liver/RBC) antibody (GTX101033) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Pyruvate Kinase (liver/RBC)(GTX101033) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min