产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Drosophila, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.67 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human RAB11B. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10619861
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
RAB11B, member RAS oncogene family , H-YPT3 , NDAGSCW
细胞定位
Cell membrane; Lipid-anchor; Cytoplasmic side
功能与背景
The Ras superfamily of small GTP-binding proteins, which includes the Ras (see MIM 190020), Ral (see MIM 179550), Rho (see MIM 165390), Rap (see MIM 179520), and Rab (see MIM 179508) families, is involved in controlling a diverse set of essential cellular functions. The Rab family, including RAB11B, appears to play a critical role in regulating exocytotic and endocytotic pathways (summary by Zhu et al., 1994 [PubMed 7811277]).[supplied by OMIM]
数据库
研究领域
產品图片
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GTX119095 ICC/IF Image
RAB11B antibody [N1C3] detects RAB11B protein by immunofluorescent analysis.Sample: DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:250.Red: Tau, a Axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX119095 IHC-P Image
RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX119095 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RAB11B antibody(GTX119095) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX119095 IHC-P Image
RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX119095 IHC-Fr Image
RAB11B antibody [N1C3] detects RAB11B Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX119095 IHC-P Image
RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX119095 IHC-P Image
RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX119095 IHC-P Image
RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.RAB11B stained by RAB11B antibody [N1C3] (GTX119095) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX119095 ICC/IF Image
GRAMD1B antibody [C1C2], Internal detects GRAMD1B protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde at RT for 15 min. Green: GRAMD1B protein stained by GRAMD1B antibody [C1C2], Internal (GTX119095) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX119095 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX119095 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX119095 WB Image
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (GTX119095) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
RAB11B antibody [N1C3]
目录号 GTX119095
Rating
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( Average 5 based on 1 users reviews)
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实验应用
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 09-May-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells
Amount used :
10 μg
Blocking :
5% skim milk in TBST, 27°C, 1Hr
Primary Antibody :
1:5000, 4°C, 12Hr
Secondary Antibody :
GTX213110-01, 1:5000, 27°C, 2Hr
条带说明 :
Two groups (compound A -/+) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody (GTX119095) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.