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RAB35抗体 [N1C3]

Orthogonal
Anti-RAB35 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120294
Anti-RAB35 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120294
Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294
Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294
Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294
Anti-RAB35 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120294
Anti-RAB35 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120294
Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294

目录号 GTX120294

文献引用 1
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat, Hamster
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 RAB35 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

23 kDa. ( Note )

预测种属反应

Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human RAB35. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10724116

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

RAB35, member RAS oncogene family , H-ray , RAB1C , RAY

细胞定位

Cell membrane; Lipid-anchor; Cytoplasmic side (Potential) , Melanosome

数据库

研究领域

產品图片

Anti-RAB35 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120294

GTX120294 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using RAB35(GTX120294) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB35 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120294

GTX120294 ICC/IF Image

RAB35 antibody [N1C3] detects RAB35 protein at Golgi apparatus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAB35 stained by RAB35 antibody [N1C3] (GTX120294) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294

GTX120294 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294

GTX120294 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294

GTX120294 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAB35 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120294

GTX120294 IHC-P Image

RAB35 antibody [N1C3] detects RAB35 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
RAB35 stained by RAB35 antibody [N1C3] (GTX120294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB35 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX120294

GTX120294 IHC-P Image

RAB35 antibody [N1C3] detects RAB35 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
RAB35 stained by RAB35 antibody [N1C3] (GTX120294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB35 antibody [N1C3] used in Western Blot (WB). GTX120294

GTX120294 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] (GTX120294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Wu B et al. Carcinogenesis 2022; 43 (11) : 1059-1070 LAMTOR1 degrades MHC-II via the endocytic in hepatocellular carcinoma.
实验应用 : WB
种属反应 : Human
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产品评论

RAB35 antibody [N1C3] 目录号 GTX120294

Rating
( Average 3 based on 1 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 3 based on 1 users reviews)
Anti-RAB35 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120294
Date : Anonymous submitted on 31-Mar-2014
Score :
Application Tested : ICC/IF
Sample Species : Hm
Sample : Chinese Hamster Ovary Cells
Blocking : 1% BSA in PBS, 37°C, 0.5Hr
Fixation : PFA, 37°C, 0.08Hr
Permeabilization /Antigen retrieval : Triton 0.5%, 37°C, 2minHr
Primary Antibody : 1:30, 37°C, 1Hr
Figure Note : This picture shows a cell in cytokinesis stained with Rab35 antibody. Rab35 (red) localises to midbody.
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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