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RAB5A抗体 [HL1497]

Recombinant antibody KO/KD
Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971
Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971
Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971
Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971
Anti-RAB5A antibody [HL1497] used in Western Blot (WB). GTX636971

目录号 GTX636971

3

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1497

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse, Rat, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 RAB5A 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

24 kDa. ( Note )

预测种属反应

Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human RAB5A. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

RAB5A, member RAS oncogene family , RAB5

细胞定位

Cell membrane,Cytoplasmic vesicle,Membrane,Cytoplasm, cytosol,Endosome membrane

数据库

研究领域

產品图片

Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971

GTX636971 IHC-P Image

RAB5A antibody [HL1497] detects RAB5A protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
RAB5A stained by RAB5A antibody [HL1497] (GTX636971) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971

GTX636971 IHC-P Image

RAB5A antibody [HL1497] detects RAB5A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat lymph node.
RAB5A stained by RAB5A antibody [HL1497] (GTX636971) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971

GTX636971 IHC-P Image

RAB5A antibody [HL1497] detects RAB5A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
RAB5A stained by RAB5A antibody [HL1497] (GTX636971) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB5A antibody [HL1497] used in IHC (Paraffin sections) (IHC-P). GTX636971

GTX636971 IHC-P Image

RAB5A antibody [HL1497] detects RAB5A protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
RAB5A stained by RAB5A antibody [HL1497] (GTX636971) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAB5A antibody [HL1497] used in Western Blot (WB). GTX636971

GTX636971 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB5A antibody [HL1497] (GTX636971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品评论

RAB5A antibody [HL1497] 目录号 GTX636971

Rating
( Average 5 based on 3 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-RAB5A antibody [HL1497] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636971
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HAP1 WT and RAB5A KO cells
Blocking : PBS, 5% BSA, 5% goat serum, 0.01% Triton X-100, 25°C, 0.5Hr
Permeabilization /Antigen retrieval : PBS, 0.1% Triton X-100, 25°C, 0.17Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : WT cells: Green dashed line KO cells: Magenta dashed line Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-RAB5A antibody [HL1497] used in Immunoprecipitation (IP). GTX636971
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 cells
条带说明 : SM: 4% starting material UB: 4% unbound fraction
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together, 30 μL of protein A-Sepharose beads. 0.5 mL aliquots at 2.0 mg/mL of lysate were incubated, an antibody-bead conjugate for ~1 hrs at 4ºC. Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-RAB5A antibody [HL1497] used in Western Blot (WB). GTX636971
Date : Anonymous submitted on 13-Sep-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and RAB5A KO cells
Amount used : 30 μg
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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