产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
A431 , HeLa , HepG2 , 293T
预测种属反应
Rat, Rabbit, Sheep, Bovine, Dog, Xenopus laevis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.4 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human RAB7A. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2886362
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
RAB7A, member RAS oncogene family , PRO2706 , RAB7
细胞定位
Late endosome , Lysosome , Cytoplasmic vesicle , phagosome , Melanosome
功能与背景
RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX130847 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB7A antibody (GTX130847) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX130847 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB7A antibody (GTX130847) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX130847 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with RAB7A antibody (GTX130847) diluted at 1:500 and competitor's antibody (sc-10767) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX130847 IHC-P Image
RAB7A antibody detects RAB7A protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
RAB7A antibody (GTX130847) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130847 IHC-P Image
RAB7A antibody detects RAB7A protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
RAB7A antibody (GTX130847) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130847 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB7A antibody (GTX130847) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
RAB7A antibody
目录号 GTX130847
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Rating
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( Average 5 based on 1 users reviews)
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实验应用
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 18-Jun-2023
Application Tested :
IHC-P
Sample Species :
Ms
Sample :
Mouse spinal cord tissue
Blocking :
0.5% skim milk in PBST, 25°C, 2Hr
Fixation :
4% PFA
Permeabilization /Antigen retrieval :
Microwave heating in citrate buffer / PBS containing Triton X-100 (PBST)
Primary Antibody :
1:1000, 4°C, 18Hr
