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RAS (G12D Mutant)抗体 [HL2640]

Recombinant antibody Overexpression
Anti-RAS (G12D Mutant) antibody [HL2640] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639096
Anti-RAS (G12D Mutant) antibody [HL2640] used in Western Blot (WB). GTX639096
Anti-RAS (G12D Mutant) antibody [HL2640] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639096

目录号 GTX639096

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2640

同种型

 IgG

实验应用

 WB, ICC/IF

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

22 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Synthetic peptide of human RAS (G12D Mutant)

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

KRAS proto-oncogene, GTPase , C-K-RAS , CFC2 , K-RAS2A , K-RAS2B , K-RAS4A , K-RAS4B , K-Ras , KI-RAS , KRAS1 , KRAS2 , NS , NS3 , RALD , RASK2 , c-Ki-ras2

细胞定位

Cell membrane,Cytoplasm, cytosol

功能与背景

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-RAS (G12D Mutant) antibody [HL2640] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639096

GTX639096 ICC/IF Image

RAS (G12D Mutant) antibody [HL2640] detects RAS (G12D Mutant) protein at cytoplasm by immunofluorescent analysis.
Sample: Aspc-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RAS (G12D Mutant) antibody [HL2640] used in Western Blot (WB). GTX639096

GTX639096 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS (G12D Mutant) antibody [HL2640] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639096

GTX639096 ICC/IF Image

Overexpression

RAS (G12D Mutant) antibody [HL2640] detects RAS (G12D Mutant) protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL2640] (GTX639096) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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