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RAS抗体

Overexpression
Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480
Anti-RAS antibody used in Western Blot (WB). GTX132480
Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480
Anti-RAS antibody used in Western Blot (WB). GTX132480

目录号 GTX132480

文献引用 8
4

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse, Rat, Zebrafish, Goat, Frog, Squirrel
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 RAS 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预测种属反应

Human (100%)| Mouse (100%)| Rat (100%)| Zebrafish (100%)| Bovine (100%)| Chicken (100%)| Japanese medaka (100%)| Xenopus laevis (100%)| Xenopus tropicalis (100%)| Rhesus Monkey (100%)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KRAS. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886657

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

研究领域

產品图片

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Ras antibody [N1N3] detects Ras protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ras antibody [N1N3] (GTX132480) diluted at a dilution of 1:1000.

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Ras antibody [N1N3] detects Ras protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ras antibody [N1N3] (GTX132480) diluted at a dilution of 1:500.

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480

GTX132480 IHC-P Image

Ras antibody detects Ras protein at cell membrane in rat urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded rat urinary bladder.
Ras antibody (GTX132480) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (GI: gastrointestinal)

Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480

GTX132480 IHC-P Image

Ras antibody detects Ras protein at cytoplasm in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Ras antibody (GTX132480) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RAS antibody used in Western Blot (WB). GTX132480

GTX132480 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Cheng CA et al. Adv Sci (Weinh) 2024; 11 (3) : e2304926 Ultrasensitive and High-Resolution Protein Spatially Decoding Framework for Tumor Extracellular Vesicles.
Hu W et al. JCI Insight 2021; 6 (3) NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.
实验应用 : WB
种属反应 : Mouse
Cui J et al. Genes (Basel) 2020; 11 (9) MiR-184 Combined with STC2 Promotes Endometrial Epithelial Cell Apoptosis in Dairy Goats via RAS/RAF/MEK/ERK Pathway.
实验应用 : WB
Zhu C et al. Animals (Basel) 2020; 10 (8) CircRNA8220 Sponges MiR-8516 to Regulate Cell Viability and Milk Synthesis via Ras/MEK/ERK and PI3K/AKT/mTOR Pathways in Goat Mammary Epithelial Cells.
实验应用 : WB
种属反应 : Goat
Liu X et al. J Cell Physiol 2020; (Epub) miR-34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ-8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways.
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产品评论

RAS antibody 目录号 GTX132480

Rating
( Average 4.5 based on 4 users reviews)
实验应用
Western Blot(WB)
( Average 4.3 based on 3 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-RAS antibody used in Western Blot (WB). GTX132480
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : MIAPaca2 cell lysates
Amount used : 10μg
Blocking : 2% BSA, 25°C, 1Hr
Primary Antibody :
Anti-RAS antibody used in IHC (Paraffin sections) (IHC-P). GTX132480
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : colon cancer tissue
Fixation : Citrate buffer, 0.3°C
Primary Antibody : 1:500, 4°C, 16Hr
Anti-RAS antibody used in Western Blot (WB). GTX132480
Date : Anonymous submitted on 17-Jun-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 60μg
Blocking : 5% milk, 24°C, 1.5Hr
Primary Antibody : 1:1200, 4°C, 16Hr
Anti-RAS antibody used in Western Blot (WB). GTX132480
Date : Anonymous submitted on 29-Apr-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC3 cells
Amount used : 30μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 24Hr
我要评论产品
Application Reference
Cheng CA et al. Adv Sci (Weinh) 2024; 11 (3):e2304926 Ultrasensitive and High-Resolution Protein Spatially Decoding Framework for Tumor Extracellular Vesicles.
Hu W et al. JCI Insight 2021; 6 (3) NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.
实验应用 : WB
种属反应 : Mouse
Cui J et al. Genes (Basel) 2020; 11 (9) MiR-184 Combined with STC2 Promotes Endometrial Epithelial Cell Apoptosis in Dairy Goats via RAS/RAF/MEK/ERK Pathway.
实验应用 : WB
Zhu C et al. Animals (Basel) 2020; 10 (8) CircRNA8220 Sponges MiR-8516 to Regulate Cell Viability and Milk Synthesis via Ras/MEK/ERK and PI3K/AKT/mTOR Pathways in Goat Mammary Epithelial Cells.
实验应用 : WB
种属反应 : Goat
Liu X et al. J Cell Physiol 2020; (Epub) miR-34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ-8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways.
Zhang Y et al. Biochim Biophys Acta 2018; 1862 (8):1721-1728 FoxO4 activity is regulated by phosphorylation and the cellular environment during dehydration in the African clawed frog, Xenopus laevis.
实验应用 : WB
种属反应 : Frog
Chen PH et al. Neuropharmacology 2016; (Epub) the chac1-inhibited notch3 pathway is involved in *-induced glioma cytotoxicity.
实验应用 : WB
种属反应 : Human
Zhang Y et al. Cryobiology 2016; 73 (2):112-9 Inhibition of skeletal muscle atrophy during torpor in ground squirrels occurs through downregulation of MyoG and inactivation of Foxo4.
实验应用 : WB
种属反应 : Squirrel
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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