产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Mouse liver , 293T , A431 , HeLa , HepG2
预测种属反应
Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.62 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human RFC4. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1951693
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
replication factor C subunit 4 , A1 , RFC37
细胞定位
Nucleus
功能与背景
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052](/templates/default/js/lightbox/images/loading.gif "Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052")
|
GTX104052 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC4 antibody [N1C3] (GTX104052) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX104052 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A549, using RFC4(GTX104052) antibody at 1:200 dilution.
|
|
|
GTX104052 WB Image
Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX104052 diluted at 1:500
|
|
|
GTX104052 IHC-P Image
Immunohistochemical analysis of paraffin-embedded MDAMB-468 Xenograft, using RFC4(GTX104052) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX104052 IHC-P Image
RFC4 antibody [N1C3] detects RFC4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded MDA-MB-231xenograft.
RFC4 antibody [N1C3] (GTX104052) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
文献引用
产品评论
目前没有使用 RFC4 antibody [N1C3] (GTX104052) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052](/upload/website/prouct_img/small/GTX104052/GTX104052_40079_20180518_WB_w_23060120_532.webp "Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052")
![Anti-RFC4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104052](/upload/website/prouct_img/small/GTX104052/GTX104052_IFA_w_23060120_450.webp "Anti-RFC4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104052")
![Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052](/upload/website/prouct_img/small/GTX104052/GTX104052_40079_WB_M_liver_w_23060120_361.webp "Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052")
![Anti-RFC4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104052](/upload/website/prouct_img/small/GTX104052/GTX104052_IHC_w_23060120_132.webp "Anti-RFC4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104052")
![Anti-RFC4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104052](/upload/website/prouct_img/small/GTX104052/GTX104052_40079_20151227_IHC-P_w_23060120_164.webp "Anti-RFC4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104052")
![Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052](/upload/website/prouct_img/normal/GTX104052/GTX104052_40079_20180518_WB_w_23060120_532.webp "Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052")
![Anti-RFC4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104052](/upload/website/prouct_img/normal/GTX104052/GTX104052_IFA_w_23060120_450.webp "Anti-RFC4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104052")
![Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052](/upload/website/prouct_img/normal/GTX104052/GTX104052_40079_WB_M_liver_w_23060120_361.webp "Anti-RFC4 antibody [N1C3] used in Western Blot (WB). GTX104052")
![Anti-RFC4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104052](/upload/website/prouct_img/normal/GTX104052/GTX104052_IHC_w_23060120_132.webp "Anti-RFC4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104052")
![Anti-RFC4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104052](/upload/website/prouct_img/normal/GTX104052/GTX104052_40079_20151227_IHC-P_w_23060120_164.webp "Anti-RFC4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104052")
