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RFP抗体 [RF5R]

Anti-RFP antibody [RF5R] used in Western Blot (WB). GTX82561

目录号 GTX82561

文献引用 4

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 RF5R

同种型

 IgG1

实验应用

 WB, IHC-P, IHC-Fr, IP, Dot, ELISA, Immunostaining

种属反应

 Species independent
产品包装
100 μg (¥4300)
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产品应用

应用说明

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000-3000
Immunostaining: Use at a dilution of 1:500 - 1:2000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

预期分子量

27 kDa. ( Note )

产品说明

Recognizes native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, tdTomato, etc.

产品属性

形式

Liquid

存储溶液

10mM PBS pH7.2

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates

纯化方式

Protein A purified

偶联

Unconjugated

RRID

AB_11178232

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

RFP tag , RFP , dsRed , red fluorescent protein

功能与背景

ince the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.

研究领域

產品图片

Anti-RFP antibody [RF5R] used in Western Blot (WB). GTX82561

GTX82561 WB Image

1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with RFP-tagged protein vector; untransfected control (1), transfected with Turbo-RFP (2), and transfected with DsRed (3)

文献引用

Application Reference

Ellis PS et al. Immun Ageing 2022; 19 (1) : 31 A subset of gut leukocytes has telomerase-dependent "hyper-long" telomeres and require telomerase for function in zebrafish.
Bertozzi A et al. Dev Biol 2020; (Epub) Is zebrafish heart regeneration "complete"? Lineage-restricted cardiomyocytes proliferate to pre-injury numbers but some fail to differentiate in fibrotic hearts.
实验应用 : IHC-Fr
Vega-Cabrera LA et al. PLoS One 2017; 12 (2) : e0172737 Analysis of Spo0M function in Bacillus subtilis.

Clone Reference

Jun S et al. Nat Commun 2016; 7:10994 LIG4 mediates Wnt signalling-induced radioresistance.
实验应用 : IHC-P
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SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4300
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