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RGS4抗体 [HL1522]

Recombinant antibody KO/KD Orthogonal
Anti-RGS4 antibody [HL1522] used in Western Blot (WB). GTX636996
Anti-RGS4 antibody [HL1522] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636996
Anti-RGS4 antibody [HL1522] used in Western Blot (WB). GTX636996
Anti-RGS4 antibody [HL1522] used in Western Blot (WB). GTX636996
Anti-RGS4 antibody [HL1522] used in IHC (Paraffin sections) (IHC-P). GTX636996
Anti-RGS4 antibody [HL1522] used in IHC (Paraffin sections) (IHC-P). GTX636996

目录号 GTX636996

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1522

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 RGS4 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

23 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human RGS4. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

regulator of G protein signaling 4 , RGP4 , SCZD9

功能与背景

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-RGS4 antibody [HL1522] used in Western Blot (WB). GTX636996

GTX636996 WB Image

KO/KD

Unboiled wild-type (WT) and RGS4 knockout (KO) SH-SY5Y cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RGS4 antibody [HL1522] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636996

GTX636996 ICC/IF Image

RGS4 antibody [HL1522] detects RGS4 protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-RGS4 antibody [HL1522] used in Western Blot (WB). GTX636996

GTX636996 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-RGS4 antibody [HL1522] used in Western Blot (WB). GTX636996

GTX636996 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-RGS4 antibody [HL1522] used in IHC (Paraffin sections) (IHC-P). GTX636996

GTX636996 IHC-P Image

RGS4 antibody [HL1522] detects RGS4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RGS4 antibody [HL1522] used in IHC (Paraffin sections) (IHC-P). GTX636996

GTX636996 IHC-P Image

RGS4 antibody [HL1522] detects RGS4 protein at cell membrane and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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产品包装 报价 (¥)
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¥ 1700
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