产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against human ROBO1 Extracellular domain.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Extracellular domain of human ROBO1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
roundabout guidance receptor 1 , DUTT1 , SAX3
细胞定位
Cell membrane,Cell projection, axon
功能与背景
Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
数据库
研究领域
產品图片
![Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936](/templates/default/js/lightbox/images/loading.gif "Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936")
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GTX638936 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody [HL2559] (GTX638936) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638936 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody [HL2559] (GTX638936) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638936 IHC-P Image
ROBO1 antibody [HL2559] detects ROBO1 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat ovary.ROBO1 stained by ROBO1 antibody [HL2559] (GTX638936) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638936 IHC-P Image
ROBO1 antibody [HL2559] detects ROBO1 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.ROBO1 stained by ROBO1 antibody [HL2559] (GTX638936) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936](/upload/website/prouct_img/small/GTX638936/GTX638936_45215_20240119_WB_Z_24012217_498.webp "Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936")
![Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936](/upload/website/prouct_img/small/GTX638936/GTX638936_45215_20231103_WB_TPM_watermark_23110819_659.webp "Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936")
![Anti-ROBO1 antibody [HL2559] used in IHC (Paraffin sections) (IHC-P). GTX638936](/upload/website/prouct_img/small/GTX638936/GTX638936_T-45138_20230829_IHC-P_R_23091319_337.webp "Anti-ROBO1 antibody [HL2559] used in IHC (Paraffin sections) (IHC-P). GTX638936")
![Anti-ROBO1 antibody [HL2559] used in IHC (Paraffin sections) (IHC-P). GTX638936](/upload/website/prouct_img/small/GTX638936/GTX638936_T-45138_20230829_IHC-P_M_23091319_951.webp "Anti-ROBO1 antibody [HL2559] used in IHC (Paraffin sections) (IHC-P). GTX638936")
![Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936](/upload/website/prouct_img/normal/GTX638936/GTX638936_45215_20240119_WB_Z_24012217_498.webp "Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936")
![Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936](/upload/website/prouct_img/normal/GTX638936/GTX638936_45215_20231103_WB_TPM_watermark_23110819_659.webp "Anti-ROBO1 antibody [HL2559] used in Western Blot (WB). GTX638936")
![Anti-ROBO1 antibody [HL2559] used in IHC (Paraffin sections) (IHC-P). GTX638936](/upload/website/prouct_img/normal/GTX638936/GTX638936_T-45138_20230829_IHC-P_R_23091319_337.webp "Anti-ROBO1 antibody [HL2559] used in IHC (Paraffin sections) (IHC-P). GTX638936")
![Anti-ROBO1 antibody [HL2559] used in IHC (Paraffin sections) (IHC-P). GTX638936](/upload/website/prouct_img/normal/GTX638936/GTX638936_T-45138_20230829_IHC-P_M_23091319_951.webp "Anti-ROBO1 antibody [HL2559] used in IHC (Paraffin sections) (IHC-P). GTX638936")
