RPA32 (phospho Thr21)抗体 [HL3692]

RPA32 (phospho Thr21) antibody [HL3692] detects RPA32 (phospho Thr21) protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPA32 (phospho Thr21) stained by RPA32 (phospho Thr21) antibody [HL3692] (GTX641864) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPA32 (phospho Thr21) antibody [HL3692] (GTX641864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.