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RPA70抗体 [C1C3]

KO/KD
Anti-RPA70 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108749
Anti-RPA70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108749
Anti-RPA70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108749
Anti-RPA70 antibody [C1C3] used in Immunoprecipitation (IP). GTX108749
Anti-RPA70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108749
Anti-RPA70 antibody [C1C3] used in Western Blot (WB). GTX108749
Anti-RPA70 antibody [C1C3] used in Western Blot (WB). GTX108749

目录号 GTX108749

文献引用 3
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 RPA70 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

68 kDa. ( Note )

阳性对照

Mouse liver , 293T , A431 , HeLa , HepG2

预测种属反应

Rat, Sheep, Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human RPA70. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1951746

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

replication protein A1 , HSSB , MST075 , REPA1 , RF-A , RP-A , RPA70

细胞定位

Nucleus

功能与背景

Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

数据库

研究领域

產品图片

Anti-RPA70 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108749

GTX108749 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit (GTX108749) antibody at 1:200 dilution.

Anti-RPA70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108749

GTX108749 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit (GTX108749) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPA70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108749

GTX108749 IHC-P Image

RPA70 antibody [C1C3] detects RPA1 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RPA70 antibody [C1C3] (GTX108749) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPA70 antibody [C1C3] used in Immunoprecipitation (IP). GTX108749

GTX108749 IP Image

Immunoprecipitation of RPA70 protein from 293T whole cell extracts using 5 μg of RPA70 antibody [C1C3] (GTX108749).
Western blot analysis was performed using RPA70 antibody [C1C3] (GTX108749).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-RPA70 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX108749

GTX108749 IHC-P Image

RPA70 antibody [C1C3] detects RPA70 protein at cytoplasm and nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary adenocarcinoma.
RPA70 antibody [C1C3] (GTX108749) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RPA70 antibody [C1C3] used in Western Blot (WB). GTX108749

GTX108749 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX108749 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPA70 antibody [C1C3] used in Western Blot (WB). GTX108749

GTX108749 WB Image

RPA70 antibody [C1C3] detects RPA70 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPA70 antibody [C1C3] (GTX108749) diluted at 1:1000.

文献引用

Application Reference

Liu MY et al. Nucleic Acids Res 2024; 52 (1) : 204-222 ATR phosphorylates DHX9 at serine 321 to suppress R-loop accumulation upon genotoxic stress.
Chen WT et al. J Biomed Sci 2021; 28 (1) : 81 Elp1 facilitates RAD51-mediated homologous recombination repair via translational regulation.
实验应用 : ICC/IF
种属反应 : Mouse
Pietro Pichierri et al. bioRxiv 2018; (Epub) RAD51 AND MITOTIC FUNCTION OF MUS81 ARE ESSENTIAL FOR RECOVERY FROM LOW-DOSE OF CAMPTOTHECIN IN THE ABSENCE OF THE WRN EXONUCLEASE.
实验应用 : WB
种属反应 : Human
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产品评论

RPA70 antibody [C1C3] 目录号 GTX108749

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-RPA70 antibody [C1C3] used in Western Blot (WB). GTX108749
Date : Anonymous submitted on 21-Aug-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP-1 cells with/without RPA70 knockout treatment
Primary Antibody : 1:1000, 4°C, 16Hr
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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