产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Drosophila, Bovine, Chicken, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909929
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
RNA polymerase II subunit B , POL2RB , RPB2 , hRPB140
细胞定位
Nucleus
功能与背景
This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
数据库
研究领域
產品图片
![Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977](/templates/default/js/lightbox/images/loading.gif "Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977")
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GTX635977 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (GTX635977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635977 WB Image
Whole zebrafish extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (GTX635977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635977 IHC-Wm Image
RPB2 antibody [HL1043] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RPB2 stained by RPB2 antibody [HL1043] (GTX635977) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC
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GTX635977 IHC-Wm Image
RPB2 antibody [HL1043] detects RPB2 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: RPB2 stained by RPB2 antibody [HL1043] (GTX635977) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC
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GTX635977 IHC-P Image
RPB2 antibody [HL1043] detects RPB2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.RPB2 stained by RPB2 antibody [HL1043] (GTX635977) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635977 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (GTX635977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX635977 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (GTX635977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635977 WB Image
293T whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with RPB2 antibody [HL1043] (GTX635977) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635977 IHC-P Image
RPB2 antibody [HL1043] detects RPB2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat lung.RPB2 stained by RPB2 antibody [HL1043] (GTX635977) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635977 IHC-P Image
RPB2 antibody [HL1043] detects RPB2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.RPB2 stained by RPB2 antibody [HL1043] (GTX635977) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977](/upload/website/prouct_img/small/GTX635977/GTX635977_44340_20221209_WB_D_C_22121123_648.webp "Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977")
![Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977](/upload/website/prouct_img/small/GTX635977/GTX635977_44340_20221216_WB_Z_22122018_706.webp "Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977")
![Anti-RPB2 antibody [HL1043] used in IHC (Whole mount) (IHC-Wm). GTX635977](/upload/website/prouct_img/small/GTX635977/GTX635977_44340_20230324_IHC-Wm_Z_1_23041023_130.webp "Anti-RPB2 antibody [HL1043] used in IHC (Whole mount) (IHC-Wm). GTX635977")
![Anti-RPB2 antibody [HL1043] used in IHC (Whole mount) (IHC-Wm). GTX635977](/upload/website/prouct_img/small/GTX635977/GTX635977_44340_20230324_IHC-Wm_Z_23041023_921.webp "Anti-RPB2 antibody [HL1043] used in IHC (Whole mount) (IHC-Wm). GTX635977")
![Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977](/upload/website/prouct_img/small/GTX635977/GTX635977_44340_20220506_IHC-P_w_23061202_410.webp "Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977")
![Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977](/upload/website/prouct_img/small/GTX635977/GTX635977_44340_20220401_WB_R_w_23061202_820.webp "Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977")
![Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977](/upload/website/prouct_img/small/GTX635977/GTX635977_44340_20220401_WB_M_w_23061202_643.webp "Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977")
![Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977](/upload/website/prouct_img/small/GTX635977/GTX635977_44340_20210611_WB_w_23061202_556.webp "Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977")
![Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977](/upload/website/prouct_img/small/GTX635977/GTX635977_44340_20221228_IHC-P_R_22122901_580.webp "Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977")
![Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977](/upload/website/prouct_img/small/GTX635977/GTX635977_44340_20221228_IHC-P_multiple_M_22122901_411.webp "Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977")
![Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977](/upload/website/prouct_img/normal/GTX635977/GTX635977_44340_20221209_WB_D_C_22121123_648.webp "Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977")
![Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977](/upload/website/prouct_img/normal/GTX635977/GTX635977_44340_20221216_WB_Z_22122018_706.webp "Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977")
![Anti-RPB2 antibody [HL1043] used in IHC (Whole mount) (IHC-Wm). GTX635977](/upload/website/prouct_img/normal/GTX635977/GTX635977_44340_20230324_IHC-Wm_Z_1_23041023_130.webp "Anti-RPB2 antibody [HL1043] used in IHC (Whole mount) (IHC-Wm). GTX635977")
![Anti-RPB2 antibody [HL1043] used in IHC (Whole mount) (IHC-Wm). GTX635977](/upload/website/prouct_img/normal/GTX635977/GTX635977_44340_20230324_IHC-Wm_Z_23041023_921.webp "Anti-RPB2 antibody [HL1043] used in IHC (Whole mount) (IHC-Wm). GTX635977")
![Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977](/upload/website/prouct_img/normal/GTX635977/GTX635977_44340_20220506_IHC-P_w_23061202_410.webp "Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977")
![Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977](/upload/website/prouct_img/normal/GTX635977/GTX635977_44340_20220401_WB_R_w_23061202_820.webp "Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977")
![Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977](/upload/website/prouct_img/normal/GTX635977/GTX635977_44340_20220401_WB_M_w_23061202_643.webp "Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977")
![Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977](/upload/website/prouct_img/normal/GTX635977/GTX635977_44340_20210611_WB_w_23061202_556.webp "Anti-RPB2 antibody [HL1043] used in Western Blot (WB). GTX635977")
![Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977](/upload/website/prouct_img/normal/GTX635977/GTX635977_44340_20221228_IHC-P_R_22122901_580.webp "Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977")
![Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977](/upload/website/prouct_img/normal/GTX635977/GTX635977_44340_20221228_IHC-P_multiple_M_22122901_411.webp "Anti-RPB2 antibody [HL1043] used in IHC (Paraffin sections) (IHC-P). GTX635977")
