产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human RPE65. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
retinoid isomerohydrolase RPE65 , BCO3 , LCA2 , RP20 , mRPE65 , p63 , rd12 , sRPE65
细胞定位
Cytoplasm,Cell membrane
功能与背景
The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital. [provided by RefSeq, Oct 2017]
数据库
研究领域
產品图片
![Anti-RPE65 antibody [HL1519] used in Western Blot (WB). GTX636993](/templates/default/js/lightbox/images/loading.gif "Anti-RPE65 antibody [HL1519] used in Western Blot (WB). GTX636993")
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GTX636993 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RPE65 antibody [HL1519] (GTX636993) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636993 IHC-P Image
RPE65 antibody [HL1519] detects RPE65 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: RPE65 stained by RPE65 antibody [HL1519] (GTX636993) diluted at 1:200.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636993 WB Image
Human tissue extract (5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RPE65 antibody [HL1519] (GTX636993) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-RPE65 antibody [HL1519] used in Western Blot (WB). GTX636993](/upload/website/prouct_img/small/GTX636993/GTX636993_44725_20220708_WB_M_eye_22071401_194.webp "Anti-RPE65 antibody [HL1519] used in Western Blot (WB). GTX636993")
![Anti-RPE65 antibody [HL1519] used in IHC (Paraffin sections) (IHC-P). GTX636993](/upload/website/prouct_img/small/GTX636993/GTX636993_T-44676_20220527_IHC-P_M_w_23061202_385.webp "Anti-RPE65 antibody [HL1519] used in IHC (Paraffin sections) (IHC-P). GTX636993")
![Anti-RPE65 antibody [HL1519] used in Western Blot (WB). GTX636993](/upload/website/prouct_img/small/GTX636993/GTX636993_44725_20240301_WB_eye_24030600_384.webp "Anti-RPE65 antibody [HL1519] used in Western Blot (WB). GTX636993")
![Anti-RPE65 antibody [HL1519] used in Western Blot (WB). GTX636993](/upload/website/prouct_img/normal/GTX636993/GTX636993_44725_20220708_WB_M_eye_22071401_194.webp "Anti-RPE65 antibody [HL1519] used in Western Blot (WB). GTX636993")
![Anti-RPE65 antibody [HL1519] used in IHC (Paraffin sections) (IHC-P). GTX636993](/upload/website/prouct_img/normal/GTX636993/GTX636993_T-44676_20220527_IHC-P_M_w_23061202_385.webp "Anti-RPE65 antibody [HL1519] used in IHC (Paraffin sections) (IHC-P). GTX636993")
![Anti-RPE65 antibody [HL1519] used in Western Blot (WB). GTX636993](/upload/website/prouct_img/normal/GTX636993/GTX636993_44725_20240301_WB_eye_24030600_384.webp "Anti-RPE65 antibody [HL1519] used in Western Blot (WB). GTX636993")
