RPL17抗体 [N1C3-2]

目录号 GTX111934

文献引用 3

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P
种属反应	Human, Rat
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX111934 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

21 kDa. ( Note )

阳性对照

HeLa , HepG2 , rat pancreas

预测种属反应

Mouse, Zebrafish, Bovine, Cat, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human RPL17 Recombinant protein.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1951755

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

ribosomal protein L17 , L17 , PD-1 , RPL23

功能与背景

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17. Two alternative splice variants have been observed, each encoding the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

数据库

Gene ID: 6139 RPL17
UniProt: P18621 RPL17

研究领域

產品图片

GTX111934 ICC/IF Image

RPL17 antibody [N1C3-2] detects RPL17 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPL17 protein stained by RPL17 antibody [N1C3-2] (GTX111934) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX111934 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
B: HepG2 (GTX27900)
12% SDS PAGE
GTX111934 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111934 IHC-P Image

RPL17 antibody [N1C3-2] detects RPL17 protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
RPL17 antibody [N1C3-2] (GTX111934) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111934 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RPL17 antibody [N1C3-2] (GTX111934) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111934 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using RPL17(GTX111934) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111934 IHC-P Image

RPL17 antibody [N1C3-2] detects RPL17 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
RPL17 antibody [N1C3-2] (GTX111934) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Dionne KL et al. J Biol Chem 2019; 294 (6) : 1944-1955 The 40S ribosomal protein uS5 (RPS2) assembles into an extraribosomal complex with human ZNF277 that competes with the PRMT3-uS5 interaction.

Landry-Voyer AM et al. Mol Cell Biol 2016; (Epub) Human PDCD2L is an export substrate of CRM1 that associates with 40S ribosomal subunit precursors.

实验应用 : WB

种属反应 : Human

Fregoso OI et al. Mol Cell 2013; 50 (1) : 56-66 Splicing-factor oncoprotein SRSF1 stabilizes p53 via RPL5 and induces cellular senescence.

实验应用 : WB

种属反应 : Human

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Application Reference

Dionne KL et al. J Biol Chem 2019; 294 (6):1944-1955 The 40S ribosomal protein uS5 (RPS2) assembles into an extraribosomal complex with human ZNF277 that competes with the PRMT3-uS5 interaction.

Landry-Voyer AM et al. Mol Cell Biol 2016; (Epub) Human PDCD2L is an export substrate of CRM1 that associates with 40S ribosomal subunit precursors.

实验应用 : WB

种属反应 : Human

Fregoso OI et al. Mol Cell 2013; 50 (1):56-66 Splicing-factor oncoprotein SRSF1 stabilizes p53 via RPL5 and induces cellular senescence.

实验应用 : WB

种属反应 : Human

SDS
Glycerol.pdf
Thimerosal.pdf

产品包装

报价 (¥)

100 μl

¥ 4000

25 μl

¥ 1700

Qty

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RPL17抗体 [N1C3-2]

目录号 GTX111934

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

功能与背景

数据库

研究领域

產品图片

GTX111934 ICC/IF Image

GTX111934 WB Image

GTX111934 IHC-P Image

GTX111934 WB Image

GTX111934 IHC-P Image

GTX111934 IHC-P Image

文献引用

Application Reference

产品评论

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