产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Mouse brain , HeLa , HepG2
预测种属反应
Rat, Drosophila, Bovine, Dog, Chicken, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2.34 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human RPS6. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2037923
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
ribosomal protein S6 , S6
功能与背景
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX113542 ICC/IF Image
RPS6 antibody detects RPS6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RPS6 stained by RPS6 antibody (GTX113542) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX113542 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 antibody (GTX113542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113542 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 antibody (GTX113542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113542 WB Image
Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113542 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113542 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RPS6(GTX113542) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113542 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using RPS6(GTX113542) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
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