产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500 - 1:2000 |
1:50 - 1:100 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
Batch dependent (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
A phospho specific peptide corresponding to residues surrounding S240/244 of human RPS6KB1
纯化方式
Purified by affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
ribosomal protein S6 , S6
功能与背景
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX66604 WB Image
WB analysis of HeLa cells treated by 10% FBS at 37ºC for 30 minutes after serum-starvation overnight and NIH/3T3 cells treated by 10% FBS at 37ºC for 30 minutes after serum-starvation overnight using GTX66604 RPS6 (phospho Ser240/Ser244) antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX66604 IP Image
IP analysis of 293T cell lysate using GTX66604 RPS6 (phospho Ser240/Ser244) antibody. Antibody amount : 3μg / 200μg lysate Dilution : 1:1000
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文献引用
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产品评论
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