Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunoprecipitation of Rad50 protein from NT2D1 whole cell extract using 5 μg of Rad50 antibody [13B3] (GTX70228).
Western blot analysis was performed using Rad50 antibody [13B3] (GTX70228).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01).

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded PC-3 xenograft.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad50 protein stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded CAL 27 xenograft.
Rad50 antibody [13B3] (GTX70228) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the journal Cell Rep in 2016. PMID: 27568553

The data was published in the journal PLoS Pathog in 2017. PMID: 28430817

The data was published in the journal Nat Commun in 2015.PMID: 26215093

The data was published in the journal J Biol Chem in 2013. PMID: 23525106

The data was published in the journal Hum Mol Genet in 2015. PMID: 26085575

The data was published in the journal PLoS One in 2012. PMID: 23285190

The data was published in the journal PLoS One in 2012. PMID: 23285190

The data was published in the journal Mol Cancer in 2010. PMID: 20509860

The data was published in the journal EMBO Mol Med in 2013. PMID: 23341130

The data was published in the journal PLoS Pathog in 2017. PMID: 28430817

The data was published in the journal BMC Biol in 2015. PMID: 25857663

The data was published in the journal Nat Commun in 2015.PMID: 26215093