产品说明
摘要
Rad50 antibody detects RAD50, a ~154 kDa protein that, together with MRE11 and NBS1, forms the MRN complex that is intimately involved in DNA damage signaling, DNA double-strand break (DSB) repair, recombination, and replication. The complex is also involved other facets of genomic homeostasis including telomere maintenance. MRN plays a pivotal role in the DNA damage response (DDR) through interactions with ATM and ATR.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HeLa , HeLa nuclear extract
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.7 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Amino acids 1-425 of Rad50 expressed in E. coli.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_372854
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
RAD50 double strand break repair protein , NBSLD , RAD502 , hRad50
细胞定位
Nucleus
功能与背景
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]
数据库
研究领域
產品图片
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GTX70228 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70228 IHC-P Image
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Rad50 stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70228 IHC-P Image
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Rad50 stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70228 IHC-P Image
Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded PC-3 xenograft. Rad50 antibody [13B3] (GTX70228) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70228 WB Image
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70228 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (GTX70228) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70228 IHC-P Image
Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded CAL 27 xenograft. Rad50 antibody [13B3] (GTX70228) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70228 ICC/IF Image
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad50 protein stained by Rad50 antibody [13B3] (GTX70228) diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
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GTX70228 IP Image
Immunoprecipitation of Rad50 protein from NT2D1 whole cell extract using 5 μg of Rad50 antibody [13B3] (GTX70228). Western blot analysis was performed using Rad50 antibody [13B3] (GTX70228). EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01).
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GTX70228 ICC/IF Image
The data was published in the journal Nat Commun in 2015.PMID: 26215093
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GTX70228 WB Image
The data was published in the journal Cell Rep in 2016. PMID: 27568553
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GTX70228 WB Image
The data was published in the journal PLoS Pathog in 2017. PMID: 28430817
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GTX70228 WB Image
The data was published in the journal BMC Biol in 2015. PMID: 25857663
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GTX70228 WB IP Image
The data was published in the journal PLoS Pathog in 2017. PMID: 28430817
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GTX70228 WB Image
The data was published in the journal EMBO Mol Med in 2013. PMID: 23341130
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GTX70228 WB Image
The data was published in the journal Nat Commun in 2015.PMID: 26215093
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GTX70228 WB Image
The data was published in the journal Mol Cancer in 2010. PMID: 20509860
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GTX70228 WB Image
The data was published in the journal PLoS One in 2012. PMID: 23285190
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GTX70228 WB Image
The data was published in the journal PLoS One in 2012. PMID: 23285190
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GTX70228 WB Image
The data was published in the journal Hum Mol Genet in 2015. PMID: 26085575
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GTX70228 WB Image
The data was published in the journal J Biol Chem in 2013. PMID: 23525106
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文献引用
产品评论
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