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Rad52抗体 [5H9]

Anti-Rad52 antibody [5H9] used in Western Blot (WB). GTX70301
Anti-Rad52 antibody [5H9] used in Western Blot (WB). GTX70301

目录号 GTX70301

文献引用 3

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 5H9

同种型

 IgG1

实验应用

 WB

种属反应

 Human
产品包装
100 μl (¥4300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

46 kDa. ( Note )

阳性对照

HeLa whole cell lysate

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Human Rad52 recombinant protein expressed in E. coli.

纯化方式

Protein G purified

偶联

Unconjugated

RRID

AB_377592

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

RAD52 homolog, DNA repair protein

细胞定位

Nucleus

功能与背景

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]

数据库

研究领域

產品图片

Anti-Rad52 antibody [5H9] used in Western Blot (WB). GTX70301

GTX70301 WB Image

Western blot experiment using Rad52 antibody (GTX70301) to detect human Rad52 protein expression in HeLa whole cell lysate

Anti-Rad52 antibody [5H9] used in Western Blot (WB). GTX70301

GTX70301 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad52 antibody [5H9] (GTX70301) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

文献引用

Application Reference

Momcilovic O et al. PLoS One 2010; 5 (10) : e13410 DNA damage responses in human induced pluripotent stem cells and embryonic stem cells.
实验应用 : WB
种属反应 : Human
de Souza-Pinto NC et al. Mol Cell Biol 2009; 29 (16) : 4441-54 The recombination protein RAD52 cooperates with the excision repair protein OGG1 for the repair of oxidative lesions in mammalian cells.
种属反应 : Human
Crosby ME et al. Cancer Res 2009; 69 (3) : 1221-9 MicroRNA regulation of DNA repair gene expression in hypoxic stress.
实验应用 : WB
种属反应 : Human
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PBS.pdf
产品包装 报价 (¥)
¥ 4300
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