形式
Liquid
存储溶液
PBS
保存剂
0.1% Sodium azide
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
浓度
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原
Keyhole limpet hemocyanin (KLH)
偶联
Biotin
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
别名
Isotype control for Rat IgG2a kappa , Rat IgG2a negative control , Rat negative control for IgG2a kappa , Rat IgG2a kappa isotype control , Rat isotype control for IgG2a kappa , Negative contr , Rat IgG2a isotype control , Rat IgG2a kappa negative control
功能与背景
Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).
