Procedure:
1. After chromogen staining, wash slide 2-3X with distilled or deiononized water.
2. Apply GTX04084 for 3-5 minutes at RT.
3. Rinse slide to be mounted with Distilled or DEIONIZED water, touch the edges of slide on a paper towel to remove excess water and place the slides on a flat surface.
4. Apply mount mounting medium directly on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Wait for 5 minutes. Excess mounting medium can be removed by touching the edges of slide against a paper towel.
5. Let stand at room temperature for about 1 to 2 hours, or the slides can be heated at 37- 40ºC for 40-60 minutes. The slides are ready for visualization under a microscope, when 10X or 20X lenses are used.
At this step for any reason if coverslip has to be removed, follow these instructions:
Soak the slide in warm (37ºC )distilled or deionized water for 5-10 minutes until the mounting medium is dissolved. Rinse slide with warm water several times to remove all mounting medium; the slide can be remounted again if required.
For Oil Immersion lens, the slide can be visualized as follows:
A drop of oil can be placed directly on dried Mounting medium for visualization. the oil can be removed by wiping with Kimwipes or lens paper.
Or apply 1-2 drops of any organic mounting medium on top of dried mounting medium, which has protected the specimen, cover slide with coverslip. The slide is ready for visualization.
