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Respiratory Syncytial virus Nucleoprotein抗体 [HL1296]

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in Western Blot (WB). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in Western Blot (WB). GTX636710
Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in Western Blot (WB). GTX636710

目录号 GTX636710

宿主

Rabbit

克隆

Monoclonal

克隆编号

HL1296

同种型

IgG

实验应用

WB, ICC/IF, ELISA, Sandwich ELISA

种属反应

Respiratory syncytial virus
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Respiratory syncytial virus

免疫原

Recombinant protein encompassing a sequence within the center region of Respiratory Syncytial virus Nucleoprotein. (Strain A2)

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2910052

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

產品图片

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710

GTX636710 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710

GTX636710 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant HEK293 expressed, N-terminal respiratory syncytial virus type A nucleoprotein (50 ng). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (160000-1.9*10-2 pM) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
3. Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709)

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710

GTX636710 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710

GTX636710 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture:Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP
Pair 2:
Capture:Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP
Pair 3:
Capture:Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.d with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710

GTX636710 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in ELISA (ELISA). GTX636710

GTX636710 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 μg/mL, respectively.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648)
Detection:
Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) conjugated with HRP
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) conjugated with HRP
Pair 3:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246 (GTX636648)
Detection:
Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) conjugated with HRP
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in Western Blot (WB). GTX636710

GTX636710 WB Image

Respiratory syncytial virus lysate (1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in Western Blot (WB). GTX636710

GTX636710 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Respiratory Syncytial virus Nucleoprotein antibody [HL1296] used in Western Blot (WB). GTX636710

GTX636710 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品评论

Respiratory Syncytial virus Nucleoprotein antibody [HL1296] 目录号 GTX636710

Rating ( Average 5 based on 2 users reviews)
实验应用
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Jun-2022
Score :
Application Tested : ICC/IF
Sample Species : RSV
Sample : HepG2 cells with/without mCherry-RSV (A Long Strain) infection, MOI 1, 24hrs
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 3% BSA and 0.1% Triton in PBS
条带说明 :
Green : Primary antibody
Dilution : 1:1000
Figure Note :
The image is from a collaborating laboratory.
Date : Anonymous submitted on 10-Jun-2022
Score :
Application Tested : WB
Sample Species : RSV
Sample : HepG2 cells with/without RSV (A Long Strain) infection, MOI 1, 24hrs
Amount used : 20μg
Blocking : 5% milk in PBS-Tween 0.02%
条带说明 : Dilution : 1:1000
Figure Note :
The image is from a collaborating laboratory.
产品包装 报价 (¥)
¥ 4000
¥ 1700
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