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S100 beta抗体 [HL2228]

Recombinant antibody Overexpression
Anti-S100 beta antibody [HL2228] used in Western Blot (WB). GTX638273
Anti-S100 beta antibody [HL2228] used in IHC (Paraffin sections) (IHC-P). GTX638273
Anti-S100 beta antibody [HL2228] used in IHC (Paraffin sections) (IHC-P). GTX638273
Anti-S100 beta antibody [HL2228] used in IHC (Paraffin sections) (IHC-P). GTX638273
Anti-S100 beta antibody [HL2228] used in IHC (Paraffin sections) (IHC-P). GTX638273
Anti-S100 beta antibody [HL2228] used in Western Blot (WB). GTX638273
Anti-S100 beta antibody [HL2228] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638273
Anti-S100 beta antibody [HL2228] used in Western Blot (WB). GTX638273

目录号 GTX638273

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2228

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

11 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human S100 beta recombinant protein

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

S100 calcium binding protein B , NEF , S100 , S100-B , S100beta

细胞定位

Cytoplasm,Nucleus

功能与背景

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-S100 beta antibody [HL2228] used in Western Blot (WB). GTX638273

GTX638273 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody [HL2228] (GTX638273) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-S100 beta antibody [HL2228] used in IHC (Paraffin sections) (IHC-P). GTX638273

GTX638273 IHC-P Image

S100 beta antibody [HL2228] detects S100 beta protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-S100 beta antibody [HL2228] used in IHC (Paraffin sections) (IHC-P). GTX638273

GTX638273 IHC-P Image

S100 beta antibody [HL2228] detects S100 beta protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-S100 beta antibody [HL2228] used in IHC (Paraffin sections) (IHC-P). GTX638273

GTX638273 IHC-P Image

S100 beta antibody [HL2228] detects S100 beta protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Green: S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 , a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-S100 beta antibody [HL2228] used in IHC (Paraffin sections) (IHC-P). GTX638273

GTX638273 IHC-P Image

S100 beta antibody [HL2228] detects S100 beta protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat hippocampus.
Green: S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 , a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-S100 beta antibody [HL2228] used in Western Blot (WB). GTX638273

GTX638273 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody [HL2228] (GTX638273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-S100 beta antibody [HL2228] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638273

GTX638273 ICC/IF Image

S100 beta antibody [HL2228] detects S100 beta protein at glia by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-S100 beta antibody [HL2228] used in Western Blot (WB). GTX638273

GTX638273 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody [HL2228] (GTX638273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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