SARS-CoV-2 (COVID-19) Nucleocapsid抗体 [HL344]

Name: SARS-CoV-2 (COVID-19) Nucleocapsid抗体 [HL344]
Brand: GeneTex
SKU: GTX635679
Price: 4000.00 RMB

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in ELISA (ELISA). GTX635679

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Western Blot (WB). GTX635679

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC (Paraffin sections) (IHC-P). GTX635679

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635679

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL344] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635679

目录号 GTX635679

文献引用 49

宿主	Rabbit
克隆	Monoclonal
克隆编号	HL344
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA, IHC-P (cell pellet)
种属反应	SARS Coronavirus 2
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 SARS-CoV-2 (COVID-19) nucleocapsid 的产品

View product citations for antibody GTX635679 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:500-1:1000
IHC-Fr	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
IHC-P (cell pellet)	Assay dependent
Note : IHC-P Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes. Sandwich ELISA Capture : GTX635679, Detection : GTX635678 / GTX635688 / GTX635686-01. Please notice that GTX635688 / GTX635678 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635679. Please contact us for custom HRP-conjugated antibody.

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

SARS Coronavirus 2

免疫原

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

纯化方式

Affinity purified by protein A.

偶联

Unconjugated

RRID

AB_2888553

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

研究领域

產品图片

GTX635679 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) and (GTX135592-pro) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635679 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635679 IHC-P Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).

The IHC-P was performed by HISTOWIZ.

GTX635679 IHC-P Image

GTX635679 WB Image

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.074 nM

GTX635679 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

GTX635679 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635679 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635679. Please contact us for custom HRP-conjugated antibody.

GTX635679 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (GTX136779-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688), and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635679 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635679 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 virus (Wild type or B.1.617.2 delta variant) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635679 IHC-P Image

The data was published in the 2022 in Sci Rep. PMID: 35264719

GTX635679 IHC-P Image

The data was published in the 2022 in Sci Rep. PMID: 35264719

文献引用

Application Reference

Tsumita T et al. Aging Cell 2024; 23 (2) : e14050 Viral uptake and pathophysiology of the lung endothelial cells in age-associated severe SARS-CoV-2 infection models.

Imbiakha B et al. Sci Adv 2024; 10 (1) : eadg5461 Adaptive immune cells are necessary for SARS-CoV-2-induced pathology.

L Zhang et al. mBio 2024; : e0307123 CRL4B E3 ligase recruited by PRPF19 inhibits SARSCoV-2 infection by targeting ORF6 for ubiquitin-dependent degradation

实验应用 : WB, IHC-P

V Castro et al. ACS Nano 2023; 17 (22) : 22708-22721 Three-Dimensional Remodeling of SARS-CoV2-Infected Cells Revealed by Cryogenic Soft X-ray Tomography

实验应用 : ICC/IF

Uemura K et al. Proc Natl Acad Sci U S A 2023; 120 (42) : e2304139120 2-thiouridine is a broad-spectrum antiviral nucleoside analogue against positive-strand RNA viruses.

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Application Reference

Tsumita T et al. Aging Cell 2024; 23 (2):e14050 Viral uptake and pathophysiology of the lung endothelial cells in age-associated severe SARS-CoV-2 infection models.

Imbiakha B et al. Sci Adv 2024; 10 (1):eadg5461 Adaptive immune cells are necessary for SARS-CoV-2-induced pathology.

L Zhang et al. mBio 2024;:e0307123 CRL4B E3 ligase recruited by PRPF19 inhibits SARSCoV-2 infection by targeting ORF6 for ubiquitin-dependent degradation

实验应用 : WB, IHC-P

V Castro et al. ACS Nano 2023; 17 (22):22708-22721 Three-Dimensional Remodeling of SARS-CoV2-Infected Cells Revealed by Cryogenic Soft X-ray Tomography

实验应用 : ICC/IF

Uemura K et al. Proc Natl Acad Sci U S A 2023; 120 (42):e2304139120 2-thiouridine is a broad-spectrum antiviral nucleoside analogue against positive-strand RNA viruses.

Imbiakha B et al. Sci Adv 2023; 9 (38):eadj1736 Age-dependent acquisition of pathogenicity by SARS-CoV-2 Omicron BA.5.

P?rez-Vargas J et al. Emerg Microbes Infect 2023; 12 (2):2246594 A novel class of broad-spectrum active-site-directed 3C-like protease inhibitors with nanomolar antiviral activity against highly immune-evasive SARS-CoV-2 Omicron subvariants.

S Andreu et al. Front Microbiol 2023; 14:1185504 Dextran sulfate from Leuconostoc mesenteroides B512F exerts potent antiviral activity against SARS-CoV-2 in vitro and in vivo

实验应用 : ICC/IF

Cloherty APM et al. Emerg Microbes Infect 2023; 12 (1):2195020 Berbamine suppresses intestinal SARS-CoV-2 infection via a BNIP3-dependent autophagy blockade.

实验应用 : ICC/IF

Li Z et al. Signal Transduct Target Ther 2023; 8 (1):53 The E3 ligase RNF5 restricts SARS-CoV-2 replication by targeting its envelope protein for degradation.

实验应用 : WB, IHC-P

Gon?alves R et al. iScience 2023; 26 (2):105972 SARS-CoV-2 variants induce distinct disease and impact in the bone marrow and thymus of mice.

P?rez-Vargas J et al. Antiviral Res 2023; 209:105484 Discovery of lead natural products for developing pan-SARS-CoV-2 therapeutics.

实验应用 : ICC/IF

Garcia G Jr et al. PLoS Biol 2022; 20 (11):e3001851 Hippo signaling pathway activation during SARS-CoV-2 infection contributes to host antiviral response.

实验应用 : WB

Shapira T et al. Mol Biomed 2022; 3 (1):43 Inhibition of glycogen synthase kinase-3-beta (GSK3β) blocks nucleocapsid phosphorylation and SARS-CoV-2 replication.

实验应用 : ICC/IF

Yaron TM et al. Sci Signal 2022; 15 (757):eabm0808 Host protein kinases required for SARS-CoV-2 nucleocapsid phosphorylation and viral replication.

实验应用 : WB

Shimizu H et al. iScience 2022; 25 (11):105314 LIGHTHOUSE illuminates therapeutics for a variety of diseases including COVID-19.

实验应用 : ICC/IF

Yamamoto Y et al. Int J Mol Sci 2022; 24 (1) Direct Inhibition of SARS-CoV-2 Spike Protein by Peracetic Acid.

Yamamoto Y et al. Int J Mol Sci 2022; 23 (24) SARS-CoV-2 Spike Protein Mutation at Cysteine-488 Impairs Its Golgi Localization and Intracellular S1/S2 Processing.

Chan JF et al. Cell Rep Med 2022; 3 (10):100774 A molecularly engineered, broad-spectrum anti-coronavirus lectin inhibits SARS-CoV-2 and MERS-CoV infection in?vivo.

Ma H et al. Cell Res 2022; 32 (9):831-842 Hetero-bivalent nanobodies provide broad-spectrum protection against SARS-CoV-2 variants of concern including Omicron.

Tirosh Shapira et al. Nature 2022; 605 (7909):340-348 A? TMPRSS2 inhibitor acts as a pan-SARS-CoV-2 prophylactic and therapeutic.

Lissenya B Argueta et al. iScience 2022; 25 (5):104223 Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy.

Naoya Kitamura et al. J Med Chem 2022; 65 (4):2848-2865 Expedited Approach toward the Rational Design of Noncovalent SARS-CoV-2 Main Protease Inhibitors.

实验应用 : ICC/IF

Ravindra Gupta et al. Res Sq 2022; SARS-CoV-2 Omicron spike mediated immune escape and tropism shift.

实验应用 : WB

Yasui F et al. Sci Rep 2022; 12 (1):4150 Infection with the SARS-CoV-2 B.1.351 variant is lethal in aged BALB/c mice.

实验应用 : IHC-P

Meng B et al. Nature 2022; (Epub) Altered TMPRSS2 usage by SARS-CoV-2 Omicron impacts tropism and fusogenicity.

实验应用 : WB

Suzuki R et al. Nature 2022; (Epub) Attenuated fusogenicity and pathogenicity of SARS-CoV-2 Omicron variant.

实验应用 : WB

Wright T et al. ACS Appl Mater Interfaces 2022; 14 (1):49-56 Photodynamic and Contact Killing Polymeric Fabric Coating for Bacteria and SARS-CoV-2.

Huan Ma et al. J Virol 2021; Potent Neutralization of SARS-CoV-2 by Hetero-bivalent Alpaca Nanobodies Targeting the Spike Receptor-Binding Domain.

实验应用 : ICC/IF

Lissenya B Argueta et al. bioRxiv 2021; SARS-CoV-2 Infects Syncytiotrophoblast and Activates Inflammatory Responses in the Placenta.

实验应用 : ICC/IF, IHC-P

Chunlong Ma et al. ACS Cent Sci 2021; 7 (7):1245-1260 Discovery of SARS-CoV-2 Papain-like Protease Inhibitors through a Combination of High-Throughput Screening and a FlipGFP-Based Reporter Assay.

实验应用 : ICC/IF

Mona Seifert et al. Elife 2021; 10 Inhibition of SARS-CoV-2 polymerase by nucleotide analogs from a single-molecule perspective.

实验应用 : ICC/IF

Jiang W et al. Cell Rep 2021; 37 (11):110112 A two-adjuvant multiantigen candidate vaccine induces superior protective immune responses against SARS-CoV-2 challenge.

实验应用 : IHC-P

Conforti A et al. Mol Ther 2021; (Epub) COVID-eVax, an electroporated DNA vaccine candidate encoding the SARS-CoV-2 RBD, elicits protective responses in animal models.

实验应用 : IHC-P

Ohno M et al. Viruses 2021; 13 (11) Abnormal Blood Coagulation and Kidney Damage in Aged Hamsters Infected with Severe Acute Respiratory Syndrome Coronavirus 2.

实验应用 : IHC-P

Garc?a-Dorival I et al. Antiviral Res 2021; 194:105167 Identification of Niemann-Pick C1 protein as a potential novel SARS-CoV-2 intracellular target.

实验应用 : ICC/IF

Asaka MN et al. JCI Insight 2021; 6 (19) Highly susceptible SARS-CoV-2 model in CAG promoter-driven hACE2-transgenic mice.

实验应用 : IHC-P

Tsuji K et al. J Toxicol Sci 2021; 46 (9):425-435 Development of alveolar and airway cells from human iPS cells: toward SARS-CoV-2 research and drug toxicity testing.

实验应用 : ICC/IF

Sasaki M. et al. mBio. 2021;:e0141521 SARS-CoV-2 Bearing a Mutation at the S1/S2 Cleavage Site Exhibits Attenuated Virulence and Confers Protective Immunity.

实验应用 : IHC-P

Sci Immunol 2021; 6 (59) Pharmacological activation of STING blocks SARS-CoV-2 infection.

实验应用 : ICC/IF

Shapira T. et al. bioRxiv 2021; (Epub) A novel highly potent inhibitor of TMPRSS2-like proteases blocks SARS-CoV-2 variants of concern and is broadly protective against infection and mortality in mice.

实验应用 : ICC/IF

Ruetalo N et al. mSphere 2021; 6 (1) Antibody Response against SARS-CoV-2 and Seasonal Coronaviruses in Nonhospitalized COVID-19 Patients.

实验应用 : WB

Eric Song et al. J Exp Med 2021; 218 (3):e20202135 Neuroinvasion of SARS-CoV-2 in human and mouse brain.

实验应用 : IHC-Fr

Sacco MD et al. Sci Adv 2020; 6 (50) Structure and inhibition of the SARS-CoV-2 main protease reveal strategy for developing dual inhibitors against M^pro and cathepsin L.

Song E et al. bioRxiv 2020; (Epub) Neuroinvasion of SARS-CoV-2 in human and mouse brain.

Sacco MD et al. bioRxiv 2020; Structure and inhibition of the SARS-CoV-2 main protease reveals strategy for developing dual inhibitors against M^pro and cathepsin L.

Heaton BE et al. bioRxiv 2020; (Epub) SRSF protein kinases 1 and 2 are essential host factors for human coronaviruses including SARS-CoV-2.

实验应用 : WB

Anand Ramani et al. EMBO J. 2020;:e106230 SARS-CoV-2 targets neurons of 3D human brain organoids.

Eric Song et al. bioRxiv 2020; (Epub) Neuroinvasive potential of SARS-CoV-2 revealed in a human brain organoid model.

实验应用 : IHC-Fr

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产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
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SARS-CoV-2 (COVID-19) Nucleocapsid抗体 [HL344]

目录号 GTX635679

宿主

克隆

克隆编号

同种型

实验应用

种属反应

产品应用

应用说明

产品说明

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

研究领域

產品图片

GTX635679 ELISA Image

GTX635679 WB Image

GTX635679 IHC-P Image

GTX635679 IHC-P Image

GTX635679 WB Image

GTX635679 ELISA Image

GTX635679 ELISA Image

GTX635679 WB Image

GTX635679 ELISA Image

GTX635679 IHC-P (cell pellet) Image

GTX635679 ELISA Image

GTX635679 ICC/IF Image

GTX635679 ELISA Image

GTX635679 ELISA Image

GTX635679 IHC-P Image

GTX635679 IHC-P Image

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