产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
产品说明
This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
SARS Coronavirus 2
免疫原
Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2888559
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP
研究领域
產品图片
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687](/templates/default/js/lightbox/images/loading.gif "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687")
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GTX635687 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (15.63-1000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
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GTX635687 ICC/IF Image
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX635687 IHC-P (cell pellet) Image
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635687 WB Image
SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635687 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.EC50 : 0.006 nM
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GTX635687 WB Image
Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635687 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635687 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献引用
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产品评论
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![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687](/upload/website/prouct_img/small/GTX635687/GTX635687_43978_20200529_ELISA_Indirect_2_w_23061202_432.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635687](/upload/website/prouct_img/small/GTX635687/GTX635687_43978_20200528_ICC_IF_SARSCoronavirus2_B_w_23061202_365.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635687](/upload/website/prouct_img/small/GTX635687/GTX635687_43999_20200724_IHC-P-FL_B_w_23061202_334.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687](/upload/website/prouct_img/small/GTX635687/GTX635687_44048_20220311_WB_SARSCoronavirus2_Ag_w_23061202_897.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687](/upload/website/prouct_img/small/GTX635687/GTX635687_43978_20200529_ELISA_Indirect_w_23061202_600.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687](/upload/website/prouct_img/small/GTX635687/GTX635687_43999_20201009_WB_SARScoronavirus2_B_w_23061202_429.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687](/upload/website/prouct_img/small/GTX635687/GTX635687_44200_20210115_WB_SARScoronavirus2_B_23052301_342.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687](/upload/website/prouct_img/small/GTX635687/GTX635687_43978_20200612_WB_multiplevirus_B_w_23061202_195.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687](/upload/website/prouct_img/normal/GTX635687/GTX635687_43978_20200529_ELISA_Indirect_2_w_23061202_432.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635687](/upload/website/prouct_img/normal/GTX635687/GTX635687_43978_20200528_ICC_IF_SARSCoronavirus2_B_w_23061202_365.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635687](/upload/website/prouct_img/normal/GTX635687/GTX635687_43999_20200724_IHC-P-FL_B_w_23061202_334.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687](/upload/website/prouct_img/normal/GTX635687/GTX635687_44048_20220311_WB_SARSCoronavirus2_Ag_w_23061202_897.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687](/upload/website/prouct_img/normal/GTX635687/GTX635687_43978_20200529_ELISA_Indirect_w_23061202_600.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in ELISA (ELISA). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687](/upload/website/prouct_img/normal/GTX635687/GTX635687_43999_20201009_WB_SARScoronavirus2_B_w_23061202_429.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687](/upload/website/prouct_img/normal/GTX635687/GTX635687_44200_20210115_WB_SARScoronavirus2_B_23052301_342.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687")
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687](/upload/website/prouct_img/normal/GTX635687/GTX635687_43978_20200612_WB_multiplevirus_B_w_23061202_195.webp "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453] used in Western Blot (WB). GTX635687")
