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SARS-CoV-2 (COVID-19) Nucleocapsid抗体

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in ELISA (ELISA). GTX135361
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in Western Blot (WB). GTX135361
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135361
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in ELISA (ELISA). GTX135361
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in ELISA (ELISA). GTX135361
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in Western Blot (WB). GTX135361

目录号 GTX135361

文献引用 17

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA, IHC-P (cell pellet)

种属反应

 SARS Coronavirus 2
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 SARS-CoV-2 (COVID-19) nucleocapsid 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Sandwich ELISA
Capture : GTX135361, Detection: GTX632269
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

This antibody detects SARS-CoV-2 nucleocapsid protein, but does not cross-react with SARS-CoV or MERS-CoV nucleocapsid proteins based on our internal testing.

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

SARS Coronavirus 2

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) nucleocapsid (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2887484

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

研究领域

產品图片

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in ELISA (ELISA). GTX135361

GTX135361 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135361 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in Western Blot (WB). GTX135361

GTX135361 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135361

GTX135361 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in ELISA (ELISA). GTX135361

GTX135361 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in ELISA (ELISA). GTX135361

GTX135361 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody used in Western Blot (WB). GTX135361

GTX135361 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135361) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

de Melo GD et al. Nat Commun 2023; 14 (1) : 4485 Neuroinvasion and anosmia are independent phenomena upon infection with SARS-CoV-2 and its variants.
Yang J et al. Nat Microbiol 2023; 8 (1) : 121-134 Fluorogenic reporter enables identification of compounds that inhibit SARS-CoV-2.
Margaroli C et al. Res Sq 2023; Type I interferon-dependent IFIT3 signaling is critical for viral clearance in airway neutrophils.
Margaroli C et al. Front Med (Lausanne) 2023; 10 : 1118024 Spatial transcriptomic profiling of coronary endothelial cells in SARS-CoV-2 myocarditis.
实验应用 : IHC-P
Wu CT et al. Cell 2023; 186 (1) : 112-130.e20 SARS-CoV-2 replication in airway epithelia requires motile cilia and microvillar reprogramming.
实验应用 : IHC-Fr
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Application Reference
de Melo GD et al. Nat Commun 2023; 14 (1):4485 Neuroinvasion and anosmia are independent phenomena upon infection with SARS-CoV-2 and its variants.
Yang J et al. Nat Microbiol 2023; 8 (1):121-134 Fluorogenic reporter enables identification of compounds that inhibit SARS-CoV-2.
Margaroli C et al. Res Sq 2023; Type I interferon-dependent IFIT3 signaling is critical for viral clearance in airway neutrophils.
Margaroli C et al. Front Med (Lausanne) 2023; 10:1118024 Spatial transcriptomic profiling of coronary endothelial cells in SARS-CoV-2 myocarditis.
实验应用 : IHC-P
Wu CT et al. Cell 2023; 186 (1):112-130.e20 SARS-CoV-2 replication in airway epithelia requires motile cilia and microvillar reprogramming.
实验应用 : IHC-Fr
Niemeyer D et al. PLoS Biol 2022; 20 (11):e3001871 SARS-CoV-2 variant Alpha has a spike-dependent replication advantage over the ancestral B.1 strain in human cells with low ACE2 expression.
Besselink G et al. Biosensors (Basel) 2022; 12 (8) Asymmetric Mach-Zehnder Interferometric Biosensing for Quantitative and Sensitive Multiplex Detection of Anti-SARS-CoV-2 Antibodies in Human Plasma.
Carlotta Pipolo et al. PLoS One 2022; 17 (4):e0266740 Evidence of SARS-CoV-2 in nasal brushings and olfactory mucosa biopsies of COVID-19 patients.
Alison E Burkett et al. Front Med (Lausanne) 2022; 9:811546 Gastrointestinal Manifestations of COVID-19 Infection: Clinicopathologic Findings in Intestinal Resections Performed at Single Institution.
实验应用 : IHC-P
Dorman LC et al. bioRxiv 2022; A type I interferon response defines a conserved microglial state required for effective neuronal phagocytosis.
实验应用 : ICC/IF
Kaufmann E et al. Cell Rep 2022; 38 (10):110502 BCG vaccination provides protection against IAV but not SARS-CoV-2.
实验应用 : IHC-P
Kung YA et al. mBio 2022;:e0271721 Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis.
实验应用 : ICC/IF
Charlotte Steenblock et al. Nat Commun 2021; 12 (1):3534 Viral infiltration of pancreatic islets in patients with COVID-19.
实验应用 : IHC-P
Xiao Y et al. Cell 2021; 184 (25):6037-6051.e14 A defective viral genome strategy elicits broad protective immunity against respiratory viruses.
实验应用 : IHC-P
Wu CT et al. Cell Metab 2021; 33 (8):1565-1576.e5 SARS-CoV-2 infects human pancreatic β cells and elicits β cell impairment.
实验应用 : WB
Margaroli C et al. Cell Rep Med 2021; 2 (4):100242 Spatial mapping of SARS-CoV-2 and H1N1 lung injury identifies differential transcriptional signatures.
实验应用 : IHC-P
Toto V et al. Arch Pathol Lab Med 2021; (Epub) Finding of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) within Placental Tissue 11 Weeks after Maternal Infection.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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