GeneTex中国官方网站
  • China

SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) 蛋白, His tag (active)

SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active). GTX02575-pro
SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active). GTX02575-pro
SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active). GTX02575-pro
SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active). GTX02575-pro
SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active). GTX02575-pro
SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active). GTX02575-pro

目录号 GTX02575-pro

实验应用

 ELISA, Functional Assay

Speciess

 SARS Coronavirus 2
产品包装
100 μg (¥7500)
查看所有 SARS-CoV-2 (COVID-19) Spike 的产品

产品应用

Observed MW

190 kDa.

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

保存剂

No preservative

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

C-terminal 8x His tagged; 16-1213 a.a. (Uniprot #P0DTC2, D614G variant). Full-length soluble with foldon trimerization motif, mutated Furin recognition site and 3 stabilising mutations (F817P, K986P and V987P), based on/modified from Hsieh et al, 2020.

表达系统

HEK293 cells

纯化方式

Purified by affinity chromatography

纯度

> 90% by SDS-PAGE

内毒素

< 1 EU/弮g (Determined by LAL assay)

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

SARS-CoV-2 Spike , COVID-19 Spike

Target Info

The original Wuhan strain of SARS CoV2 virus has become quickly replaced by its more transmissible variant, mainly determined by a single amino acid point mutation D614G of Spike protein.

数据库

研究领域

產品图片

SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active). GTX02575-pro

GTX02575-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (D614G variant) protein, His tag (active) (GTX02575-pro), and SARS-CoV-2 spike (trimer) protein (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (13111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active). GTX02575-pro

GTX02575-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (D614G variant) protein, His tag (active) (GTX02575-pro), and SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active). GTX02575-pro

GTX02575-pro Image

Differential scanning fluorimetry (DFS) analysis the stability and the heparin binding ability. DSF of proteins were performed in the absence or presence of heparin (10 μM). The scanning fluorimetry data of the two batches shows the protein is both stable and functional, with heparin binding activity.
A and C : Melting curve of spike protein.
B and D : First derivative of the melting curves of spike to show its melting temperature as peak.
A and B : the first batch of spike protein (PBS)
C and D : the second batch of spike protein (PBS, 20% glycerol).

SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active). GTX02575-pro

GTX02575-pro Image

CD spectra of different cation forms of spike protein. The scanning fluorimetry data of the two batches shows the protein is both stable and functional, with heparin binding activity.
A and C : CD spectra were recorded on J-1100 spectrometer between 180 and 260 nm of spike protein (0.5 mg/ml).
B and D : Secondary structure were analysed by program CDSSTR of A and C, respectively.
A and B : the first batch of spike protein (PBS buffer).
C and D : the second batch of spike protein (PBS, 20% glycerol).

SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active). GTX02575-pro

GTX02575-pro Image

SDS-PAGE of GTX02575-pro SARS-CoV-2 (COVID-19) Spike (D614G variant) protein, His tag.

SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active). GTX02575-pro

GTX02575-pro Image

Gel filtration analysis of 2 different batches of spike protein.
The first batch : spike protein in PBS buffer.
The second batch : spike protein in PBS, 20% glycerol.

文献引用

目前没有使用 SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active) (GTX02575-pro) 的文献被收录。快来回报我们第一篇参考文献吧！
我要回报文献

产品评论

目前没有使用 SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active) (GTX02575-pro) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
产品包装 报价 (¥)
¥ 7500
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com