SARS-CoV-2 (COVID-19) Spike RBD抗体 [GT5449]

目录号 GTX636042

宿主	Mouse
克隆	Monoclonal
克隆编号	GT5449
同种型	IgG2a
实验应用	WB, ELISA, Sandwich ELISA
种属反应	SARS Coronavirus 2
产品包装	100 μl (¥4000), 25 μl (¥1700)

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说明书

View product citations for antibody GTX636042 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX635807, Detection : GTX636042 or Capture : GTX636042, Detection : GTX635807 / GTX635693 / GTX635713 / GTX635654. Please notice that the capture antibody needs to be conjugated to HRP to function as the detection antibody when paired with the detected antibody. Please contact us for custom HRP-conjugated antibody.

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

SARS Coronavirus 2

免疫原

Full length SARS-CoV-2 (COVID-19) Spike RBD recombinant protein. (strain:Wuhan-hu-1)

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2909940

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

研究领域

產品图片

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL)

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL)

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL)

GTX636042 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX636042 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX636042 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

GTX636042 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S1 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

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产品包装

报价 (¥)

100 μl

¥ 4000

25 μl

¥ 1700

Qty 客制化偶联服务

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SARS-CoV-2 (COVID-19) Spike RBD抗体 [GT5449]

目录号 GTX636042

宿主

克隆

克隆编号

同种型

实验应用

种属反应

产品应用

应用说明

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

研究领域

產品图片

GTX636042 ELISA Image

GTX636042 ELISA Image

GTX636042 ELISA Image

GTX636042 ELISA Image

GTX636042 ELISA Image

GTX636042 WB Image

GTX636042 ELISA Image

GTX636042 ELISA Image

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