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SARS-CoV-2 (COVID-19) Spike RBD抗体

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Western Blot (WB). GTX135385
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135385
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Western Blot (WB). GTX135385

目录号 GTX135385

文献引用 4

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF

种属反应

 SARS Coronavirus 2
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 SARS-CoV-2 (COVID-19) Spike 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

SARS Coronavirus 2

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 Spike (S1) (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2887486

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

研究领域

產品图片

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Western Blot (WB). GTX135385

GTX135385 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike antibody (GTX135385) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135385

GTX135385 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike antibody detects SARS-CoV-2 (COVID-19) Spike protein by immunofluorescent analysis.
Sample: Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike stained by SARS-CoV-2 (COVID-19) Spike antibody (GTX135385) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody used in Western Blot (WB). GTX135385

GTX135385 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135385) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Mu?oz-Al?a M? et al. mBio 2024; 15 (2) : e0292823 Surface-modified measles vaccines encoding oligomeric, prefusion-stabilized SARS-CoV-2 spike glycoproteins boost neutralizing antibody responses to Omicron and historical variants, independent of measles seropositivity.
K Yamamoto et al. Jpn J Appl Phys 2023; SARS-CoV-2 detection by using graphene FET arrays with a portable microfluidic measurement system
Mu?oz-Al?a M? et al. bioRxiv 2022; Surface-modified measles vaccines encoding oligomeric, fusion-stabilized SARS-CoV-2 spike glycoproteins bypass measles seropositivity, boosting neutralizing antibody responses to omicron and historical variants.
Andrea Estefan?a Portales et al. Life Sci 2022; 293 : 120284 ACE2 internalization induced by a SARS-CoV-2 recombinant protein is modulated by angiotensin II type 1 and bradykinin 2 receptors.
实验应用 : ICC/IF
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PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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