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SARS-CoV-2 (COVID-19) Spike RBD 蛋白, His tag (active)

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

目录号 GTX01546-pro

文献引用 2

实验应用

 ELISA, Functional Assay

Speciess

 SARS Coronavirus 2
产品包装
100 μg (¥6700)
查看所有 SARS-CoV-2 (COVID-19) Spike 的产品

产品应用

应用说明

Activity was measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 (COVID-19) Spike RBD protein at 2 μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 8-80 ng/mL.

产品属性

形式

Lyophilized powder

存储溶液

Reconstitute with distilled water to 0.1-0.5mg/ml. Lyophilized from 0.22μm-filtered PBS (pH7.4).

保存剂

No preservative

存放说明

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

Arg319-Phe541 of SARS-CoV-2 (COVID-19) Spike RBD protein (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.

表达系统

HEK293 cells

纯度

> 95% by SDS-PAGE

内毒素

< 0.1 EU/μg (determined by LAL method)

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

SARS-CoV-2 Spike , COVID-19 Spike

数据库

研究领域

產品图片

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (4.5-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.5 μg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1000-1.19*10-4 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 0.24 μM

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635792. Please contact us for custom HRP-conjugated antibody.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1000-1.19*10-4 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 0.2 μM

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, mouse Fc tag (UK variant) (GTX136024-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13.72-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL), and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) and SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (160-1.91*10-5 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 0.08 μM

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (13111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Image

SDS-PAGE of GTX01546-pro SARS-CoV-2 (COVID-19) Spike RBD protein (active).

文献引用

Application Reference

TW Liu et al. J Ethnopharmacol 2024; 320 : 117412 Polymethoxyflavone from Citrus depressa as an inhibitor against various variants of SARS-CoV-2 spike protein
Ganneru B. et al. iScience 2021; 24 (4) : 102298 Th1 skewed immune response of whole virion inactivated SARS CoV 2 vaccine and its safety evaluation.
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产品包装 报价 (¥)
¥ 6700
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